I stepped a slant of Pacman up to a 1L starter on a stir plate, and then once it was finished, I cold crashed it, decanted the starter beer, and then poured the remaining slurry into a sanitized Ball jar. After a few hours of settling, there was a pale layer of yeast on the bottom, and a darker layer of other stuff on top of it. I've seen pictures of yeast in jars after building up a starter or washing, and none of them look like this. I'm more curious than worried... This is still getting pitched into an ESB. (Although someone else's, not mine.) Does anyone know what's going on here?
The most interesting thing is that before it went into the jar, there was just one uniform pale layer at the bottom of the starter flask.