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-   -   Starter from dregs (http://www.homebrewtalk.com/f163/starter-dregs-359610/)

stillbrewin 10-08-2012 07:19 AM

Starter from dregs
 
I am trying to figure out if I have enough pitchable yeast in a starter that I made from the dregs of a st bernardus pater 6. I pitched the dregs into 100ml on a stir plate and let it go for 24hrs. I then stepped it up to 400ml then to 1000 ml. I never really got a kreusen to form on it because of the vortex from the stir I assume. I took it off the stir plate and cold crashed it. After a day in the fridge I had about 1/2 inch of yeast settled on the bottom of the 1000 ml flask. I left it in the fridge for a couple more days because I needed to pick up some more dme. I just decanted it and put it in a 2 liter flask and started the stir plate up again. I was planning on using this on a 5 gallon batch right around 1.047. How will I know how much yeast is in this starter?

sweetcell 10-08-2012 07:50 AM

so did you step it up once more to 2000 ml, or is 1000 ml your last step?

tough thing about stepping up dregs is that you can't be sure of how many cells you have in there unless you have some fancy lab equipment.

based on my estimates (i've recently stepped up dregs, brewing tomorrow), a 2 liter starter should get you in the ball park. one liter probably isn't enough.

donovanneb 10-08-2012 03:34 PM

The gravity on this is in the 40's so you might be ok, it will just be a slow starter and could take 2-3 days to get going. Culturing bottle dregs also needs to be a careful process. Not sure if the stir plate in the beginning was too harsh on the yeast. If you want to be safe go another step up, if you want to experiment and see if it is ok, then pitch now.

stillbrewin 10-08-2012 09:39 PM

stepped up again
 
I stepped it up to 2l last night. Hoping to brew tomorrow. Does 1/2 inch of yeast settled out in a 1l flask sound about right? Guess I will feel better with like an inch in the 2l flask.

stillbrewin 10-12-2012 10:58 PM

so far so good
 
Brewed on tue. no activity on wed. Nice kreusen starting on thur. Tonight giant funky kruesen 3/4 of an inch thick. Funky, smelly, and bringing clumps of czech saaz to the top. It's this brownish, greenish monster : )

I brewed this batch low gravity. I am hoping to step up the gravity, and pitch right on to the the yeast cake next batch.

I am really hoping I got the right belgian yeast for this recipe from these dregs.

If not at least I managed to step up from dregs succesfully.

sweetcell 10-13-2012 01:28 AM

what's the recipe, and what are the dregs from?

stillbrewin 10-13-2012 08:52 AM

The recipe is based on the NB patersbiere. Not an exact copy of it. 5 gal batch.

9lbs Belgian pilsner
1lb Carafoam

1oz german tradition at 60
1oz czech saaz @ 10

dry hopped 1 oz czech saaz

dregs from St Bernardus Pater 6

Pugalicious 12-05-2012 03:56 AM

How is this one going? Finished yet? Interested to hear how it did. I'm looking to do the same with a Abt 12 clone. Thanks!

beerspitnight 12-05-2012 11:25 AM

I am brewing a batch with WPL008 while I am home over Christmas. Due to the limited availability of yeast in China, I would like to bring back a couple of bottles of the brew and harvest the yeast from them. I am curious to see how this worked out for you.

stillbrewin 12-09-2012 10:57 AM

The brew went well! I am glad I decided to step up two 2 liters. It took about 36 hours for things to really get going. I got a really nice krausen by the third day.


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