As yeast viability goes down and the vial gets closer to the best before date, would increasing the length of time in the starter or a bigger starter do any good to increase total cell count? Or would you have to start, clean, make next starter be the way to get the cell count up to where it needs to be? I have a vial that is getting close to their BB date. I am going to do a 2L starter. I usually let it spin for 18-24 hours before pitching. Would any extra spinning time or bigger starter do anything for the cell count?