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Old 04-09-2014, 05:22 PM   #721
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Default Why the purified water?

I notice that the author lists bottled water as the water source. Why is this? If the medium is being boiled to such a temp, why is the purified water need? Is the bottled water used for the medium only, or is it also used as the water the fills the pressure cooker too? Thank you.

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Old 04-09-2014, 05:26 PM   #722
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Hollis, I was wondering if that was the case too for me. The first attempt left me with watery slants, so I upped the agar. Now they seem to hard. I'm wondering if my yeast growth is thin because of that.

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Old 05-13-2014, 09:03 PM   #723
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Just wanted to share a picture of my 10ml vial that I just innoculated with yeast from my slant..nice krausen forming after 20 hours or so..

1400011412842.jpg  
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Old 05-13-2014, 09:52 PM   #724
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Just wanted to share a picture of my 10ml vial that I just innoculated with yeast from my slant..nice krausen forming after 20 hours or so..
Would you mind posting your process to get to here? Did you use an inoculation loop to take colonies from a slant and put it in the vial with 10ml of sterile wort?

Also, when filling the vial, do you use canned starter that has been pre-sterilized? Or do you fill the vial, then sterilize it?
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Old 05-14-2014, 12:57 AM   #725
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Would you mind posting your process to get to here? Did you use an inoculation loop to take colonies from a slant and put it in the vial with 10ml of sterile wort?

Also, when filling the vial, do you use canned starter that has been pre-sterilized? Or do you fill the vial, then sterilize it?
Sure. I usually make about 1000ml of starter wort on the stove top in a sauce pan. Dissolve the DME, than divide it up between about 10 vials 10ml each, and two beakers one with 90 ml of wort and one with 400 ml of wort. Than I autoclave all of the vials and beakers to get sterile wort in each. I usually make 10 vials that way I can have sterile ones handy, and if I let them sit for a few days I can guarantee they are sterile.

When I am ready to make a starter for a beer, such as this one, which is a nut brown ale that I will be brewing thursday. I take a loop from my slant of wlp004, inoculate the 10 ml vial, loosen the cap to let out the co2. Let that go for 24 hours or so, enough time to verify you have an active starter. Pic of the krausen is what you want. Than pitch that 10 ml into the 90 ml for a total of 100ml. Let that go for 24 hours. I'll post pics later. Than you will pitch that into the 400 ml of starter for 500ml total. Than go to 1000ml, and then so on until you have the proper pitching amount.
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Old 05-14-2014, 06:19 AM   #726
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So do you just select a single colony from the slant to inoculate with? Do you then re-seal the slant to reuse at a later time?


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Old 05-14-2014, 10:55 AM   #727
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The first thing I do is take a loop from either the smack pack or White labs vial. Whichever yeast I am using. Than I streak a plate with the loop and let that grow for a few days to maybe a week, than I take a single colony from the plate and streak a slant. Let the slant go for a few days, unsealed. Than I cap it and put it in the fridge. When I want to use the yeast I just pull a full loop from the slant. My slants have some pretty good growth on them. I reseal the vial and put back in the fridge. I haven't gotten to the point were I need to transfer from slant to slant..

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Old 05-14-2014, 04:27 PM   #728
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Stupid question but do you aerate your tubes or beakers?


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Old 05-14-2014, 07:44 PM   #729
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No, I just shake them periodically..

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Old 05-14-2014, 08:38 PM   #730
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Here is the 100ml beaker that I dumped the 10ml into..about 15 hours in..

1400096283479.jpg  
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