Slanting yeast - Page 32

pngaudioguy · 12-28-2011, 11:17 AM

+1 to Jason. edit: (regarding 2L flask in addition for starter sizes)

Also, I bought two of the plastic racks. That way I can leave one in the fridge with my current vials in it, and the other is available for putting in the pressure cooker / storing empties in a safe way in between samples, etc.

chuckjaxfl · 12-28-2011, 01:14 PM

Quote:

Originally Posted by ZBrewski

Well my Cynmar order is looking to be about $75, did I forget anything? I already have a 1L Flask:

11527910 CLEAR GLASS VIALS w/AUTOCLAVABLE CAP, 24ml 12/PK X2 US$17.00

11520035 5 PIECE BEAKER SET, BOROSILICATE GLASS, 50ml-1000ml-PKG 035 US$18.50

15025994 PLASTIC TEST TUBE RACK, WHITE, HOLDS 24 - 25mmTUBES US$4.25

11533371 PYREX RIBBED FUNNEL, SHORT STEM, 65mm##CL US$7.30

CIM89101 DEHYDRATED AGAR POWDER, 4 oz US$18.00

13225101 NICHROME INOCULATING LOOP, 25 GAUGE WIRE, 8" TOT LENGTH
US$1.50

12030895 GLASS ALCOHOL LAMP w/CAP, 4oz, US$3.75

I'd add some petri dishes, for plates.

Edited to add: I also wanted some small Erlenmeyers. You can put 100ml in a 1L, but the stirbar seems to work better for me if the flask is 25%-75% full.

**Saccharomyces** · 01-05-2012, 02:27 AM

Quote:

Originally Posted by storunner13

If Sacc is still lurking this thread (or anyone else has tried it)...

Did you ever get around to culturing any Bug Blends? Agar/Dextrose media either by slants, or separating the strains on plates? If so, how did you make starters?

When I remember to lurk. These days I'm usually just hanging out in the chat room on IRC.

So far I have only slanted brett. It slants just like saccharomyces yeast, it just looks quite a bit different -- the colonies grow up with funny looking fractal shapes that are very neat looking.

**edecambra** · 01-09-2012, 11:50 PM

Quote:

Originally Posted by Saccharomyces

When I remember to lurk. These days I'm usually just hanging out in the chat room on IRC.

So far I have only slanted brett. It slants just like saccharomyces yeast, it just looks quite a bit different -- the colonies grow up with funny looking fractal shapes that are very neat looking.

So I traded a HBT'er for White's Yeast book and read most of it this holiday. They suggest swiping the top of your slants for yeast and punching down into the media for bacterial strains with your loop or paper clip or whatever.

I would highly suggest that book too it describes the process of plating and selecting appropriate healthy colonies for propagation which can be very useful, in good detail too.

**Westel** · 01-24-2012, 03:00 AM

Just wanted to say thanks Sacc for the great info just got my slants done can't wait to try my hand at this just waiting on my loop.

**kappclark** · 01-24-2012, 09:17 PM

Nice ! That's looks like you'll have plenty of nice first-generation yeast this year ...I got the exact same rack from cynmar

I still need to get the canner (got a computer for christmas instead of a canner )

Accidic · 01-25-2012, 04:19 AM

I get shredded husks in a moderate amount with my floating plate style model. That said, I do let the lhbs grind the 1lb increment grains (i do some self roasting frequently) and add rice hulls if it seems necessary. I've honestly only had issues with tannins with multi temp mashes with over 3hrs mash time. Also to be up front, I don't brew to compete but rather to taste.

FredTheNuke · 02-24-2012, 03:20 AM

Pardon if I missed it - been reading 32 plus pages. The original guide shows 2.5 grams of Agar (or half a bar). What is the agar agar recipe for powdered Agar Agar (one packet of gelatin is 0.25oz - which equates to roughly 7 grams). 2.5 grams seems light.?.?.?

Accidic · 02-24-2012, 11:28 AM

I used the strands at first til they got wet. When I went to get more they only had the powder (white phone). @2.5g it makes a really near rock solid product so I dropped it down to 2.1 (i want to say someone was using 1.8 actually but not sure what type) and had no issues. I think others here have used the powder without that problem so I would try the base recipe first. If it's too solid then go with the lesser. Either way, a little goes a long way.

FredTheNuke · 02-24-2012, 02:18 PM

So the four 2oz bottles I bought for $25.00 are gonna last a LONG LONG time...