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Old 01-06-2010, 09:15 PM   #101
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Don't worry about drying your slants. You're just risking infection. My slants have some liquid condensate but it doesn't interfere with yeast growth. After inoculation of a slant you should replace the cap but do not tighten to seal. Let incubate for 3-5 days at ~80F until satisfactory yeast growth occurs, then seal tight, put in a plastic bag, and refrigerate. The reason for the incubation period is to build a colony and expose contaminants.

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Old 01-12-2010, 04:03 AM   #102
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So I noticed that my slant I just made have about a tiny drop water in them. I think they'll be okay.

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Old 01-26-2010, 03:41 AM   #103
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Wanted to show you guys the slants I made with the help of this thread. Im leaning one out on the shelf to see if anything grows. I hope not. Thanx again this place rocks
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Old 01-26-2010, 03:50 PM   #104
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Krops13

Good pics... And nice use of the coat hanger to make your slant. It looks like that angle gave you a lot of surface area inside the tube.

It doesn't look like your slants are in glass. Is this true?

Did you run them through the pressure cooker?

If they are autoclavable, what was your source.

Thanks, Jason.

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Old 01-26-2010, 05:31 PM   #105
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They are glass. I prossesed them at 13-14lbs for 15 minutes. I pit a plastic plate in there with the slants but it melted.

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Old 01-31-2010, 05:11 AM   #106
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If you let the vials cool a little too long, such that the Agar/DME solution starts to solidify before it is stored at an angle, can you reheat the vials in the pressure cooker for another 15 minutes to return it to a liquid form? Or should you empty the vials and start over?

Thanks!

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Old 01-31-2010, 05:27 AM   #107
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place in water, bring to near boil or so, and re-set at desired angle. but don't remove the caps! i just re-slanted the angle on my 24 in-stock so i got as much surface area as allowed without touching the lid area.

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Old 01-31-2010, 09:52 PM   #108
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Thanks! I reheated them this morning and they returned to a nice liquid form. The vials are now sitting at an angle forming nice slants.

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Old 02-04-2010, 01:04 AM   #109
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Thanks for the write-up.

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Old 02-08-2010, 03:24 AM   #110
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i finally got a cheap alcohol lamp on ebay.. one that can sit at 30*, 60*, and 90*. what a difference it makes vs a candle..... no more carbon deposit on my nichrome loops.

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