I did this with one 22 oz bottle of DFH Squall. I made up a batch of about 1.04-1.05 wort and added a small amount of the wort straight to the bottle. Waited a few days, added some more, waited a few days, put into fridge, decanted off liquid and then pitched another small starter the night before brewing. After pitching to fermentor, fermentation started by morning. Good luck.