Tinga

Recipes 

I'm thinking about building an incubator for a couple different things. Does anyone know the best temperature to grow both sacc. and brett. at? I'm aware that they grow just fine at room temperature but I know they grow better at higher temps I'm just wondering what the best temp is to grow yeast on an agar media.

ipsiad

Recipes 

Not sure about Brett, but Sacc in general grows best around 25°C/77°F. The exact optimum temp is probably strain-dependent - different yeasts evolved to grow in different climates.

Tinga

Recipes 

you got a source on that? I'm having trouble finding a good consistent answer.

PoppinCaps

Recipes 

In the lab, we grew Sacch at 30C, and that doesn't cause any mutations. most strains crank up at that temp, despite optimum brewing temps.

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mewithstewpid

Recipes 

Poppin, with 5% co2 as well?

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Tinga

Recipes 

I'm hoping on agar with 02

PoppinCaps

Recipes 

No need for 5% CO2, that's for pH controlled growth media for liquid cultures. You're good with ambient air on agar. You can easily grow these at room temp, but an incubator will significantly speed growth.

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mewithstewpid

Recipes 

Ah nice I wasn't sure if it was the same conditions for bacteria or not

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ipsiad

Recipes 

I'm speaking from personal experience, but I'll see if I can find a source. Lab strains of Sacc grow great at 30°C, but I tried growing brewing strains at that temp and my viability went down (this is in liquid medium, btw, not plates). I got the best results in our non-heated shaker, which holds steady at 24-25°C.

PoppinCaps

Recipes 

Interesting. I never grew them in liquid at that temp, only on plates for quick culturing. Maybe that's a factor.

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