Originally Posted by EvilGnome6
Funny, I was looking to find an alternative to washing yeast for reuse. I brew a lot of high gravity ales so taking it from the primary isn't really an optimal process for me. This is what I'm trying right now:
Make 600 mL starter from a vial/smack pack
Let it ferment out and settle.
Decant 300 mL.
Seal 100 mL each in two 4 oz. Jelly Jars.
Save the Jelly Jars in the fridge for future starters.
Use remaining 100mL to make a 1000 mL starter to pitch into wort.
Next time I need the yeast, I can start the process over using yeast from one of the 100 mL samples I saved in the Jelly Jars.
Basically I do this on a larger scale:
I make a 2.5 liter starter and, after shaking/swirling well, I pour a pint into a sterilized pint mason jar. Then I cold crash, decant the liquid from the remaining 2 liter starter and pitch the slurry.
I refrigerate the mason jar, and use the slurry from it for my next 2.5 liter starter. So far, the yeast has been good up to two or three months in the fridge. I don't really plan on going over three months refrigerated or 4-5 generations.