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Old 06-25-2010, 12:32 AM   #1
passedpawn
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Default Old frozen yeast viability = good

Another point of data for those yeast freezing bankers out there...

Wyeast 3068, frozen 13 months, took right off from a 10ml tube frozen with glycerin. I fermented out 500ml on my stirplate, it bloomed in 1 day.

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Old 06-25-2010, 12:47 AM   #2
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Whoosh always good to hear that it can work for an extended period of time. I'm just about to start doing yeast freezing myself.

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Old 06-25-2010, 03:22 AM   #3
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What was your glycerin/water/yeast ratio? I just froze some yeast (wlp029) and I unfroze one after 2 weeks and it took off after 1 day, but 13 months is good to hear. I mixed 5 ml of 15% glycerin/water with 5 ml of thick yeast slurry.

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Old 06-25-2010, 03:33 AM   #4
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Actually, 15ml tubes. about 50% yeast slurry, 25% water, 25% glycerin. Roughly what you did. No worries.

BTW, I've re-animated many frozen yeasts with nary an issue.

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Old 06-25-2010, 05:07 AM   #5
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Default WOW!!! Good to know.

I have been harvesting and reusing my yeast since I started brewing. I only buy yeast at this point when I try new styles. But I now have many, many more mutchkins of yeast in my fridge than I can brew firkin of beer. I'll have to lookinto this more closly.

I so love pitching a pint of yeast in my wort and having it go at it only hours later.

But this thread has me thinking of the future

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