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Home Brew Forums > Home Brewing Beer > Fermentation & Yeast > Is my 5 month old WLP001 vial still viable?
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Old 02-05-2014, 11:09 PM   #1
Hovik
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Default Is my 5 month old WLP001 vial still viable?

Hi HBT,
I bought a vial of WLP001 Cal Ale yeast last fall and have kept it in the dark fridge ever since. I think it's "best before date" is Jan 02, 2014, making the production date Sept 02, 2013. I am making an Imperial Stout this weekend that calls for that strain but am debating as to whether or not to use the vial I have or get a fresh one. I made a 1.6L starter (4oz of DME) and have it on the stir plate. There is definitely yeast activity and I was thinking to step it up by decanting and doing another 1.6L starter. When I plug in the strain and age, Beersmith tells me I'm at like 29.6Billion cells without starter, and 177.2Billion cells with my 1.6L starter on stir plate, if I step it up with one more 1.6L dose of wort I should be right around the cell count for pitching called for by beersmith (313 Billion). However if I check the brewersfriend calculator it tells me that vial has 0% viability! Any ideas?


Thanks.

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Old 02-05-2014, 11:12 PM   #2
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If theres yeast activity then it obviously doesnt have 0% viability. Sounds like its working fine with your starters. Keep doing what you're doing.

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Old 02-05-2014, 11:14 PM   #3
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Originally Posted by casesensative View Post
If theres yeast activity then it obviously doesnt have 0% viability. Sounds like its working fine with your starters. Keep doing what you're doing.
Do you think another 1.6L step-up will bring me to the neighborhood of 313 billion cells?
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Old 02-06-2014, 12:31 AM   #4
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Do you think another 1.6L step-up will bring me to the neighborhood of 313 billion cells?
hard telling if you don't know the initial yeast count but i would keep stepping.
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