I made a 1.5 liter starter using WLP653 (Brett Lambicus) about 60 hours ago and I'm not seeing any activity at all. I did the starter like all other 40+ starters I have done before, but this time I'm not seeing any progress.
The starter has been kept at 70-75 and I even added a little yeast nutrient just in case. The vial did seem to have very little sediment, but I figured this was normal. The vial is dated best before 7/8/10, so seems like it should be viable.
I have done both C. and B. strain starters before like this without problems.
Any idea what might be happening? Bad yeast, need more time, more nutrients, more warmth?
Only other thing worth mentioning is that I am using a stir plate.
Keg1: Belgian IPA
Keg2: Wee Heavy
Keg3: Vienna Lager
Secondary: Rye Wine, Gluten Free Saison
Aging: Bourbon Barrel Imperial Stout w/ Brett, 2009 Lambic, Kriek, Framboise, 2010 Lambic, 2011 Lambic