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Old 12-27-2013, 01:01 AM   #1
timrox1212
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Default Frozen yeast

So long story short my yeast washes got frozen. All are 2-3rd washes of yeast in the mason jars with the liquid still on top. Am I screwed or is there hope?



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Old 12-27-2013, 01:12 AM   #2
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How long did they freeze for? Did the slurry freeze solid, or just a bit slushy?
I've had vials of yeast freeze before, and it's always survived enough that I could revive it with a starter.



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Old 12-27-2013, 10:07 AM   #3
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It's still frozen like totally solid. I didn't want to unfreeze it and damage it.

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Old 12-27-2013, 01:56 PM   #4
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Gotcha. Best bet is to thaw it slowly, and feed it to a starter. If any yeasties make it through the freeze, you'll find out w the starter.

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Old 12-27-2013, 02:06 PM   #5
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Agreed I would make a small weak starter and see if you get any growth. If it is totally frozen, all the yeast may be dead. But the only way to be sure is to try a starter.

You may need to take gravity samples to determine if any fermentation takes place. I have had some show little to no visible signs but did propagate.

If there is any action in the small starter then you can step up to a proper sized starter.

I, for sure would give it a try before just tossing it and buying new yeast.

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Old 12-27-2013, 06:03 PM   #6
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They're probably fine. Yeast are resilient. There've been a few threads about this lately and the yeast seem to survive. I've been interested in the topic since if I order liquid yeast this time of year there's a good chance they're getting cold enough to freeze at some point during transport. You probably just lost some viability. Someone posted recently that according to Chris White you only lose 10 percent each time they freeze.

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Old 12-28-2013, 01:34 PM   #7
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So would you recommend using 2 jars worth of yeast in a starter or stick to just one like usual? I have 4 jars of 3 different yeast that all froze so I have a few to spare if it'll work better.

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Old 01-01-2014, 03:43 PM   #8
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FYI just an update. I let the yeast dethaw for a day and pitched 1 jar into a 1/2 gallon starter. Took off like a rocket and was bubbling by around 10 hours. So I have to believe that freezing really dosent damage anything. I haven't pitched into the wort yet but starter is going strong



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