I left the pack in the fridge to slowly thaw over night and then took it out around 7am this morning (~12hrs in the fridge). It's been sitting out for about 3 hours now and has actually puffed up a fair bit, enough to spread the bottom fold by about 1/2" or so. I haven't broken the nutrient pillow yet either.
I'm not entirely sure what this means, but I'm going to assume something is still kicking in there. I think I'll activate the package around noon and see how much swelling I get by the evening.
So here's the plan:
If this package gets completely inflated, I'll go for a 1L starter and follow it with a second 1L starter resulting in over 300B cells even if the initial viability is ~50%. If not, I think I'll go for a 1.5L starter at the second stage which will get me to 300B cells even if the initial viability is around 33%.
This leaves me with a question about pitching rate: What sort of impact would over-ptiching by 30-50B cells have on the final product?