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Old 04-11-2011, 05:49 PM   #1
JeffStewart
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Default first starter for yeast bank

So I've got my starter in the fridge that I'm going to collect yeast from for my bank and my question is.. how long does it need to stay in the fridge to get the yeast out of suspension?

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Old 04-11-2011, 06:17 PM   #2
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I usually leave mine for a day or two in the fridge. After a day I would say you are probably fine but I have had a little better success with 48 hrs as it seems to settle more and compact the yeast at the bottom.

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Old 04-11-2011, 06:44 PM   #3
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Ok, thanks...

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Old 04-13-2011, 05:43 PM   #4
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So I just transfered the yeast from the starter into culture tubes. How thick should the solution be?

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Old 04-14-2011, 01:34 PM   #5
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I'm assuming that since you're using solution you're also not slanting the yeast on agar. In that case you should be using RO water so the yeast don't have anything to munch on.

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Old 04-14-2011, 02:36 PM   #6
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Yea no slants. The solution is the yeast and solids with a bit of the starter from the bottom. But in the freezer they shouldn't be eating anything anyways right? I"ve got about 9 15ml culture tubes with varying amount of solids in the bottom. Anywhere from 3ml to the entire tube. I probably did it incorrectly just trying to figure out what and correct it for next time.

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Old 04-14-2011, 03:09 PM   #7
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If you're going to be freezing them, then they need to be in a glycol solution so the cell walls of the yeast don't rupture when they thaw. I have no experience with that, but I think it needs to be a 15% glycol solution.

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Old 04-14-2011, 03:12 PM   #8
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Yea, 15% of glycerol was added and thoroughly mixed in the solution. I guess I'll just have to wait and see which ones work out and which don't.

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Old 04-14-2011, 05:04 PM   #9
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Buy the yeast book by Chris White. It has awesome information about every way to store yeast. It's cheap and will last a lifetime of brewing.

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Old 04-14-2011, 05:27 PM   #10
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http://www.homebrewtalk.com/f13/guid...st-bank-35891/

That is a post in the forums regarding doing a frozen yeast bank. In the later pages someone posted a process in which they use that I follow instead of the OP.
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