JeffStewart

Recipes 

So I've got my starter in the fridge that I'm going to collect yeast from for my bank and my question is.. how long does it need to stay in the fridge to get the yeast out of suspension?

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- Jeff

br1dge

Recipes 

I usually leave mine for a day or two in the fridge. After a day I would say you are probably fine but I have had a little better success with 48 hrs as it seems to settle more and compact the yeast at the bottom.

JeffStewart

Recipes 

Ok, thanks...

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- Jeff

JeffStewart

Recipes 

So I just transfered the yeast from the starter into culture tubes. How thick should the solution be?

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- Jeff

DrawTap88

Recipes 

I'm assuming that since you're using solution you're also not slanting the yeast on agar. In that case you should be using RO water so the yeast don't have anything to munch on.

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Fermenting: Nothing
Secondary: Nothing
Bottled: Oatmeal Porter, Double Chocolate Chipotle Porter
Kegged: IPA, Red Rye

JeffStewart

Recipes 

Yea no slants. The solution is the yeast and solids with a bit of the starter from the bottom. But in the freezer they shouldn't be eating anything anyways right? I"ve got about 9 15ml culture tubes with varying amount of solids in the bottom. Anywhere from 3ml to the entire tube. I probably did it incorrectly just trying to figure out what and correct it for next time.

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- Jeff

DrawTap88

Recipes 

If you're going to be freezing them, then they need to be in a glycol solution so the cell walls of the yeast don't rupture when they thaw. I have no experience with that, but I think it needs to be a 15% glycol solution.

__________________
Fermenting: Nothing
Secondary: Nothing
Bottled: Oatmeal Porter, Double Chocolate Chipotle Porter
Kegged: IPA, Red Rye

JeffStewart

Recipes 

Yea, 15% of glycerol was added and thoroughly mixed in the solution. I guess I'll just have to wait and see which ones work out and which don't.

__________________
- Jeff

suprchunk

Recipes 

Buy the yeast book by Chris White. It has awesome information about every way to store yeast. It's cheap and will last a lifetime of brewing.

USMCYoder

Recipes 

http://www.homebrewtalk.com/f13/guide-making-frozen-yeast-bank-35891/

That is a post in the forums regarding doing a frozen yeast bank. In the later pages someone posted a process in which they use that I follow instead of the OP.

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SEMPER SLOSH BREWING
On Deck: Wild Farmhouse Saison, Christmas Saison, Chocolate Orange Ale
Primary: Air
Secondary:Air
Drinking/Drank: Pale Ale, Sweet Saison x3, Chocolate Milk Stout, Maple Porter, Hoppy Belgian IPA, Belgian Wit, Blood Orange Wit(3rd NHC), Belgian Quad x2


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