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-   -   first starter for yeast bank (http://www.homebrewtalk.com/f163/first-starter-yeast-bank-239070/)

JeffStewart 04-11-2011 05:49 PM

first starter for yeast bank
 
So I've got my starter in the fridge that I'm going to collect yeast from for my bank and my question is.. how long does it need to stay in the fridge to get the yeast out of suspension?

br1dge 04-11-2011 06:17 PM

I usually leave mine for a day or two in the fridge. After a day I would say you are probably fine but I have had a little better success with 48 hrs as it seems to settle more and compact the yeast at the bottom.

JeffStewart 04-11-2011 06:44 PM

Ok, thanks...

JeffStewart 04-13-2011 05:43 PM

So I just transfered the yeast from the starter into culture tubes. How thick should the solution be?

DrawTap88 04-14-2011 01:34 PM

I'm assuming that since you're using solution you're also not slanting the yeast on agar. In that case you should be using RO water so the yeast don't have anything to munch on.

JeffStewart 04-14-2011 02:36 PM

Yea no slants. The solution is the yeast and solids with a bit of the starter from the bottom. But in the freezer they shouldn't be eating anything anyways right? I"ve got about 9 15ml culture tubes with varying amount of solids in the bottom. Anywhere from 3ml to the entire tube. I probably did it incorrectly just trying to figure out what and correct it for next time.

DrawTap88 04-14-2011 03:09 PM

If you're going to be freezing them, then they need to be in a glycol solution so the cell walls of the yeast don't rupture when they thaw. I have no experience with that, but I think it needs to be a 15% glycol solution.

JeffStewart 04-14-2011 03:12 PM

Yea, 15% of glycerol was added and thoroughly mixed in the solution. I guess I'll just have to wait and see which ones work out and which don't.

suprchunk 04-14-2011 05:04 PM

Buy the yeast book by Chris White. It has awesome information about every way to store yeast. It's cheap and will last a lifetime of brewing.

USMCYoder 04-14-2011 05:27 PM

http://www.homebrewtalk.com/f13/guide-making-frozen-yeast-bank-35891/

That is a post in the forums regarding doing a frozen yeast bank. In the later pages someone posted a process in which they use that I follow instead of the OP.


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