Ss Brewing Technologies Giveaway!

Home Brew Forums > Home Brewing Beer > Fermentation & Yeast > Easiest way to start an ongoing yeast culture from dry yeast?
Reply
 
LinkBack Thread Tools
Old 07-10-2010, 01:09 AM   #1
Symbiote
Feedback Score: 0 reviews
Recipes 
 
Join Date: Jun 2010
Location: Somewhere between the computers and the coffee pot.
Posts: 99
Likes Given: 3

Default Easiest way to start an ongoing yeast culture from dry yeast?

n00b here.

I have a packet of dry ale yeast and would like to turn it into half a dozen starters.

What do you recommend as the most efficient way to go about this?

__________________

Primary 1: Experimental Apfelwein

Primary 2: Experimental Apfelwein

US politics is like pro wrestling, with a nuclear option.

Symbiote is offline
 
Reply With Quote Quick reply to this message
Old 07-10-2010, 01:45 AM   #2
peoplesbrewingcoop
Feedback Score: 0 reviews
Recipes 
 
Join Date: Jun 2010
Location: Houston, TX
Posts: 41
Liked 1 Times on 1 Posts

Default

First make some media. Use whatever you can find. Some people just use malt extract. You can go to your local homebrew store to get media for a starter. I make my own use 3g malt extract, 10g dextrose, 3g yeast extract, 5g peptone in 1 Liter of water.

Put the media into jars. Since you want to make starters I am assuming you want to have enough yeast to pitch directly from these. You will need to calculate the amount of cells needed to pitch. Use Mr. Malty's Pitching Rate Calculator. The problem with the calculator is that it takes it assumes manufactured liquid or dry yeast or from a slurry. You have none of these so you will have to be creative to estimate the number of cells. You need "about 1.5 million for a lager and 0.75 million for an ale." If you want to get technical you can try to do a Yeast Plate Count, but that might be more work that you want to do. If you want to try you can PM me and I will go more into depth on how to do. I was going to write up a guide in the wiki so you could give me the jump start to do so.

Now sterilize the media that is in the jars. If you have access to an autoclave (you or a friend has access to a lab) then use that, if not go with a pressure cooker or just put the jars in a covered pot of a small amount of boiling water. Keep the lids slightly open, you dont want the pressure to crush the jars. If boiling do for 1 hour. Let the jars cooll down. Then using aseptic technique add the yeast. For you I would take your pack of dried yeas and wipe around the entire surface of the pack, keep your surface area clean and disinfected with bleach, and if you have them put on gloves. Now open your pack and sprinkle a little into you jars/flasks/ect and cover with sterilized foil (sterilize the foil with the media).

Let them grow for about 3 days and shake 3 to 4 times a day if you can. Then when you are ready then pitch. Like I said earlier, it is hard to make sure you are pitching the correct amount of yeast without a real measurement. If you are using US-05 it gets to about 200 billion cells in 1.5 L of my media in 2 days. Every yeast has a different growth rate based on the temperature, media, and other variables.

If you want consistent efficient results then buy 6 dry yeast packs. But if you want to have fun then culture your own yeasts. If you have any questions just send me a PM.

__________________

Last edited by peoplesbrewingcoop; 07-10-2010 at 01:57 AM.
peoplesbrewingcoop is offline
 
Reply With Quote Quick reply to this message
Old 07-10-2010, 01:46 AM   #3
ksbrain
Feedback Score: 0 reviews
Recipes 
 
Join Date: Feb 2007
Location: Mystic, CT
Posts: 1,018
Liked 11 Times on 11 Posts
Likes Given: 2

Default

Brew a batch and harvest the resulting yeast into six separate containers - maybe 4oz each. Follow the yeast rinsing sticky process.

__________________
ksbrain is offline
 
Reply With Quote Quick reply to this message
Reply



Quick Reply
Message:
Options
Thread Tools


Similar Threads
Thread Thread Starter Forum Replies Last Post
Will this yeast culture work? GLWIII Fermentation & Yeast 3 09-28-2011 12:32 AM
Pitching yeast from the top of an ongoing fermentation?? xbattlex Fermentation & Yeast 8 03-22-2010 05:10 PM
Some questions about Yeast culture origins Bibtm Fermentation & Yeast 1 03-16-2010 02:14 PM
Sierra Nevada yeast culture jaketothemax1000 Fermentation & Yeast 4 11-22-2009 08:47 PM
Yeast culture method? jldc Fermentation & Yeast 3 10-08-2009 08:32 PM