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Home Brew Forums > Home Brewing Beer > Fermentation & Yeast > Do you know how to make a yeast starter? Then why not farm yeast and freeze it?
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Old 11-12-2012, 05:56 AM   #321
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I would probably put it in a canning jar and boil the jar in water. Boiling 100 % glycerol is probably not a good idea and boiling diluted glycerol will make it hard to know its final concentration. This will be more controllable in a canning jar.

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Old 11-12-2012, 06:06 AM   #322
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What if you boil the water, then mix that (cooled enough to work with) with the glycerine?

I really didn't want to pick up a pressure cooker JUST for this.

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Old 11-12-2012, 07:09 AM   #323
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If you trust the sterility of the glycerol.

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Old 11-12-2012, 07:58 AM   #324
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I'm buying glycerol in drugstore and it is sterile, if you're not sure just boil it for few minutes with foil on top (mixed w/water).

What is about v/v vs. w/v dilutions?
I've been using w/v but now I'm not sure about it.. on other hand it depends on what % of gycerin/water solution we use.
I did a little math and it seems that 12% v/v dilution equals to 15% w/v.

When searching about this topic I found article where 10% v/v (12% w/v) is used, samples are frozen to -80°C:
http://nbimcc.org/JCC/2002/JCC312.pdf

Do you think there should be difference in mixing percentage depending on storage temperature (eg -20 vs -80 °C)?

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Old 11-12-2012, 02:10 PM   #325
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Old 11-13-2012, 04:47 AM   #326
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Would this glycerine be good for freezing yeast? not sure after reading the material safety sheet. The price is pretty good.
http://www.newdirectionsaromatics.ca...995-p-540.html

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Old 11-13-2012, 05:29 AM   #327
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Quote:
Originally Posted by strongale28 View Post
Would this glycerine be good for freezing yeast? not sure after reading the material safety sheet. The price is pretty good.
http://www.newdirectionsaromatics.ca...995-p-540.html
Just depends on whether you trust the source or not. The certificate of analysis is certainly vague. At least you know it tastes sweet.
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Old 11-14-2012, 07:44 PM   #328
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Apparently styrofoam coolers are NOT a good choice for holding isopropyl alcohol. I had a styrofoam cooler filled with isopropanol in the freezer since the weekend and when I came back to it today all the isopropanol had leaked out and made a huge mess. I had a somewhat similar thing happen the first time I froze cells but I thought my cooler just had a hole in it. Now I'm pretty sure the isopropanol breaks down the styrofoam and leaks out.

For freezing cells, use a PLASTIC cooler filled with isopropanol to slow down the cooling rate. DO NOT USE STYROFOAM!!!

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Old 11-14-2012, 07:52 PM   #329
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Quote:
Originally Posted by Forkhead View Post
Apparently styrofoam coolers are NOT a good choice for holding isopropyl alcohol. I had a styrofoam cooler filled with isopropanol in the freezer since the weekend and when I came back to it today all the isopropanol had leaked out and made a huge mess. I had a somewhat similar thing happen the first time I froze cells but I thought my cooler just had a hole in it. Now I'm pretty sure the isopropanol breaks down the styrofoam and leaks out.

For freezing cells, use a PLASTIC cooler filled with isopropanol to slow down the cooling rate. DO NOT USE STYROFOAM!!!
LOL. Man, you seem to be hitting a few hurdles there Forkhead. Exploding vials and now melting coolers. At least you're getting those mistakes out of the way for everybody else. Should be downhill from here right?
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Old 11-14-2012, 07:56 PM   #330
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Haha, yeah it's all part of the process. Smooth sailing from here on out (fingers crossed).

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