Ss Brewing Technologies Giveaway!

Home Brew Forums > Home Brewing Beer > Fermentation & Yeast > Do you know how to make a yeast starter? Then why not farm yeast and freeze it?
Reply
 
LinkBack Thread Tools
Old 09-19-2011, 03:23 PM   #11
ReverseApacheMaster
Registered User
Feedback Score: 0 reviews
Recipes 
 
Join Date: Jul 2009
Location: Keller, Texas
Posts: 4,887
Liked 229 Times on 192 Posts

Default

I have some yeast cultures frozen in my parents' chest freezer. I haven't pulled one to see if it is viable but I assume as long as they never thawed they should be fine. I'll have to pull a sample next time I visit and see how it works out.

__________________
ReverseApacheMaster is offline
 
Reply With Quote Quick reply to this message
Old 09-19-2011, 03:54 PM   #12
BBL_Brewer
HBT_SUPPORTER.png
Feedback Score: 0 reviews
 
BBL_Brewer's Avatar
Recipes 
 
Join Date: Feb 2011
Location: Kokomo, IN
Posts: 3,698
Liked 425 Times on 295 Posts
Likes Given: 321

Default

Quote:
Originally Posted by ReverseApacheMaster View Post
I have some yeast cultures frozen in my parents' chest freezer. I haven't pulled one to see if it is viable but I assume as long as they never thawed they should be fine. I'll have to pull a sample next time I visit and see how it works out.
I'm willing to bet that they are still alive.
__________________
Slots Down Brewery
Stick with the plan....not the sparge.


Never Ending Liquid Yeast - How to Farm Yeast and Freeze it.

BBL_Brewer is offline
 
Reply With Quote Quick reply to this message
Old 10-01-2011, 06:06 PM   #13
BBL_Brewer
HBT_SUPPORTER.png
Feedback Score: 0 reviews
 
BBL_Brewer's Avatar
Recipes 
 
Join Date: Feb 2011
Location: Kokomo, IN
Posts: 3,698
Liked 425 Times on 295 Posts
Likes Given: 321

Default

Just a small update. I just got done freezing my yeast for the year and decided to do a little qualitative testing while I was at it. I used three different strenght glycerine solutions this time. 35% 50% and 60%. My plan is to see if different levels of glycerine affect the lag time when making the first step of starter from frozen yeast. If more glycerine = shorter lag time then I will assume that more glycerine = more viable yeast surviving the freeze. If I observe no appreciable difference, then I will stay with my current assumption that the yeast are just sluggish after freezing and need more time to regain activity. If Brewitt does some viability assays for us and contributes that information, then we should have a pretty good idea of what to expect from freezing yeast at home.

__________________
Slots Down Brewery
Stick with the plan....not the sparge.


Never Ending Liquid Yeast - How to Farm Yeast and Freeze it.

BBL_Brewer is offline
 
Reply With Quote Quick reply to this message
Old 10-01-2011, 06:52 PM   #14
BeerMe21
Feedback Score: 0 reviews
Recipes 
 
Join Date: Oct 2010
Location: Costa Mesa, CA
Posts: 81
Liked 2 Times on 2 Posts
Likes Given: 1

Default

Thank you for the great write up! This is very tempting, hehehe....

__________________
BeerMe21 is offline
 
Reply With Quote Quick reply to this message
Old 10-01-2011, 07:03 PM   #15
mredge73
HBT_SUPPORTER.png
Feedback Score: 0 reviews
 
mredge73's Avatar
Recipes 
 
Join Date: May 2011
Location: La Porte, TX
Posts: 621
Liked 32 Times on 32 Posts

Default

Got my pressure cooker this week with some glycerine.
Just finished testing it by canning 7 qts of water and pressure cooking it (I will now have sterile water on hand for a while). I also did a 50:50 glycerine/water in 2x 4oz jars (only filled halfway, so yeast can occupy the other half). Next weekend I will be bottling and I plan on freezing washed yeast.

Will 25% glycerin be enough to keep them viable?

__________________
mredge73 is offline
 
Reply With Quote Quick reply to this message
Old 10-01-2011, 07:08 PM   #16
BBL_Brewer
HBT_SUPPORTER.png
Feedback Score: 0 reviews
 
BBL_Brewer's Avatar
Recipes 
 
Join Date: Feb 2011
Location: Kokomo, IN
Posts: 3,698
Liked 425 Times on 295 Posts
Likes Given: 321

Default

Try it and find out. I'm guessing it will since I've heard reports of people using 15%.

__________________
Slots Down Brewery
Stick with the plan....not the sparge.


Never Ending Liquid Yeast - How to Farm Yeast and Freeze it.

BBL_Brewer is offline
 
Reply With Quote Quick reply to this message
Old 10-01-2011, 07:33 PM   #17
WortMonger
United States Mashtronaut
HBT_LIFETIMESUPPORTER.png
Feedback Score: 0 reviews
 
WortMonger's Avatar
Recipes 
 
Join Date: Nov 2006
Location: Edmond, OK, Oklahoma
Posts: 3,141
Liked 28 Times on 27 Posts
Likes Given: 3

Default

Awesome writeup and I look forward to following this post.

__________________
"Beer... Nutritious and Delicious!"

"It's like a 15.5 gallon Mr. Beer!"
WortMonger is offline
 
Reply With Quote Quick reply to this message
Old 10-03-2011, 05:28 AM   #18
Brewitt
Senior Member
HBT_SUPPORTER.png
Feedback Score: 0 reviews
 
Brewitt's Avatar
Recipes 
 
Join Date: Jun 2011
Location: Encinitas, CA
Posts: 817
Liked 66 Times on 60 Posts
Likes Given: 9

Default

Quote:
Originally Posted by BBL_Brewer View Post
If Brewitt does some viability assays for us and contributes that information, then we should have a pretty good idea of what to expect from freezing yeast at home.
Sorry for the delay in doing some experimentation on this. Work has been quite busy and I have had no time for side projects. I will prep a starter this week and set up some frozen preparations at a few glycerin concentrations from 15 to 60% and at -30C and -80C. It will probably be a couple weeks before I know anything about viability since it requires cell counts and colony counts.
__________________
Brewitt is offline
 
Reply With Quote Quick reply to this message
Old 10-03-2011, 05:44 AM   #19
diS
HBT_SUPPORTER.png
Feedback Score: 0 reviews
Recipes 
 
Join Date: Apr 2011
Location: Croatia
Posts: 1,018
Liked 45 Times on 43 Posts
Likes Given: 12

Default

That would be great!
I had good results with 30% glycerin solution, and I think that success with smaller concentration will depend on how well is glycerin mixed with slurry.. but experiment will give more relevant informations about that.

__________________
diS is offline
 
Reply With Quote Quick reply to this message
Old 10-03-2011, 06:13 AM   #20
el_caro
Feedback Score: 0 reviews
Recipes 
 
Join Date: Feb 2011
Location: , Australia
Posts: 606
Liked 33 Times on 28 Posts
Likes Given: 8

Default

Quote:
Originally Posted by dis View Post
i had good results with 30% glycerin solution
+1
__________________
el_caro is offline
 
Reply With Quote Quick reply to this message
Reply



Quick Reply
Message:
Options
Thread Tools


Similar Threads
Thread Thread Starter Forum Replies Last Post
Mocktoberfest (marzen)- should i make a yeast starter with harvested yeast? mhayden37 Fermentation & Yeast 12 02-22-2012 01:24 PM
Use Dry Yeast or make Starter? beitzjr Fermentation & Yeast 23 04-06-2011 12:58 AM
When to make a yeast starter? mudhen5 Fermentation & Yeast 5 02-05-2011 11:18 PM
How Little Yeast to Make a Starter? Bmorebrew Fermentation & Yeast 4 06-16-2010 03:05 AM
How to make a yeast starter blackheart Fermentation & Yeast 4 05-26-2010 05:29 AM