I would love to hear from someone with experience in this.. I have been keeping yeasts on slants and plates for several months now but I have a new developing problem that I do not know the reason for.
All the batches of plates and slants I have made recently have way too much condensation on them. In the case of my plates, I am waiting for the agar to solidify after autoclaving, then I am storing them upside down in the fridge in plastic bags. When I go to use some plates, there is always now water on the MEDIA side of the plate. If I try to streak it, the yeast just gets all wet and doesn't create tight colonies.
In the case of my slants, there is enough water in them that once I streak them, the yeast is traveling down underneath the slant via the water, and causing the entire agar bulk in the slant to slide up and out of the vial because of co2 pressure.
What the heck am I doing wrong? I can't figure out what is different now from when i was having so much success.
How do you deal with condensation issues on your agar media? Is refrigeration causing this?
Thanks in advance