This is a batch of Wyeast 3787 trappist high gravity I made from the yeast stuck to the walls of my flask after pitching the first starter. What I did is after pitching my 2 liter starter I added 1 liter of 1.020 wort, let that go for 2 days, decanted it added 2 liters of 1.040 wort and after 2 days I cold crashed it. I decanted the liquid on top leaving just enough to fill this mason jar. The jar is about 2.25"X6" tall. It seems I got very good growth giving what I started with.
My question is what is above and below the line? The bottom stuff should be yeast as its creamy white. The top is much darker. All that was in it is boiled DME, energizer and nutrient. I allways thought that the yeast would be on top.