Originally Posted by Drifter
I have successfully created slants and grew the colonies and pitched them. Here's my question I made 6 slants from an original WYeast slap -pack. Now I am down to one slant, what is the proper way to make more slants with this last slant.
Make more slants, and use your noc loop to inoculate the new slants from the old. As always, use your sterile-as-possible technique, flaming/quenching, etc.
Purists might suggest that you plate out the culture from the last slant into a petri dish first, grow that, then inoculate the new slants. This might be indicated if you are concerned about the health or purity of that last culture. I've had good success both ways.
I just recultured a slant that was 8mos old and it took off fine on the plate.