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Old 11-13-2008, 09:00 PM   #1
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Default Yeast starter and stir plate

So I finally got around to getting a stir plate for yeast starters. My question is, how much do you actually run the stir plate? Is it an on/off type thing to get things going with an occasional stir, or continual for a certain amount of time? I tried searching but didn't seem to have much luck. I'm trying to get my yeast counts up without straining the little critters.

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Old 11-13-2008, 10:40 PM   #2
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It's a continuous stirring operation, usually for 12 - 18 hours.

-a.

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Old 11-13-2008, 10:46 PM   #3
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I suggest running it continuously at a speed so that the vortex is about halfway down your total volume...so for an 800ml starter the vortex is ~3" down in a 1000ml flask. Obviously a larger flask would be better, for more surface area, but continuous for the whole fermentation is the key.

I am building a stir plate tomorrow, and that's how I plan to run it. This is how we grow bacteria in our lab, and it work tremendously better than our shaker plates.

have fun,

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Old 11-13-2008, 11:53 PM   #4
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Thanks for the replies.

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Old 11-14-2008, 01:23 AM   #5
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I run mine as fast as possible from the start right until I crash cool. Hasn't seemed to have any negative affects so far.

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Old 11-14-2008, 08:58 PM   #6
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I just run it fulltime with the yeasties riding the twirl-a-whirl.

The first step up (agar slant culture in a 50ML flask) takes 18-24 hrs. Then subsequent steps move increasingly faster as cell counts increase geometrically ("they tell two friends, and THEY tell two friends, and so on, and so on...")

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Old 11-14-2008, 09:01 PM   #7
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Quote:
Originally Posted by fratermus View Post
("they tell two friends, and THEY tell two friends, and so on, and so on...")
Okay, you just dated yourself!!
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Old 11-14-2008, 09:37 PM   #8
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Just in case you were interested :
This is what you are trying to emulate with your stir plate
A fermentor or bioreactor:

http://cgi.ebay.ca/NEW-BRUNSWICK-BIOFLO-110-FERMENTOR-FERMENTER-BIOREACTOR_W0QQitemZ310100238186QQcmdZViewItemQQpt ZLH_DefaultDomain_0?hash=item310100238186&_trksid= p3286.c0.m14&_trkparms=72%3A1215|66%3A2|65%3A12|39 %3A1|240%3A1318

Sorry I couldn't find a video of one in action with a continuous whirlpool and air or oxygen bubbling in at the same time. Most of these also have pumps that can introduce various nutrients etc. at a set rate.

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Old 11-14-2008, 11:13 PM   #9
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I run mine continuously for 24 hours. When I do a 2 liter starter, I have gone 48 hours, but it is apparent that the starter wort is spent, and the yeasties are ready for another meal.

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