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Old 08-27-2007, 01:03 AM   #1
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Default Yeast Harvesting

Just finished following the instructions I found on this site for washing yeast in three easy steps. It's simple and the five yeasties are now resting in the fridge for later deployment. I have used the full yeast cake with trub for the last two batches without difficulty, but the cleaner yeast will be better, I'm sure.

I will use a starter for the next five batches and continue to use the 3B oxygenation system for the quickest and shortest lag time.

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Old 08-27-2007, 01:29 AM   #2
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Welcome to the world of saving money.

Be sure to get some of that yeast into a beer bottle and cap it if it's gonna be a while before you can use it.

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Old 08-27-2007, 02:31 AM   #3
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I always plan my brews so that I am repitching the yeast, but I've wondered how long can the yeast remain viable if you pour it into bottles, cap them and store them in the fridge.

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Old 08-27-2007, 02:56 AM   #4
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Add some glycerin (30-33%) and store in the freezer. That's what I'm doing (for the 1st time).

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Old 08-27-2007, 04:29 AM   #5
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More converts...gotta love it!!!

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Old 08-27-2007, 03:32 PM   #6
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Quote:
Originally Posted by Iordz
I always plan my brews so that I am repitching the yeast, but I've wondered how long can the yeast remain viable if you pour it into bottles, cap them and store them in the fridge.
I did a Kolsch last weekend with a 3-month old bottle. Turned out fine.

I have total confidence in any yeast I have that is less than 6 months old. Haven't gone longer than 6 months yet so can't really say.
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Old 08-27-2007, 05:34 PM   #7
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Default Yeast Harvesting - Pictures.

Here are some pics from my first venture into yeast freezing:

First yeast harvest using 8oz SunnyD containers. The didn't reseal well, so I bagged and heat sealed 'em.

A Closeup of the first batch showing the yeast settled. It's difficult to make out since the bags fog instantly when removed.

Eight 32ml (20x150mm) vials of yeast. This is from the same primary that yielded the SunnyD bottles. I split the original yield from the yeast washing and put 32oz in the SunnyD bottles, and 32oz in the fridge to floccate and be xferred when the vials arrived (today). Today, I siphoned off all the liquid and re-placed it with a 33% distilled water/glygerin mixture. That's what you see in the vials.

The yeast vials loaded into my "anti-defrost" bag to protect them from the defrost cycles.

Same bag, without the ice pack.

Same bag again, from the outside. I think we paid only $4.99 for the bag before it served as a lunch carrier for a few years. If you don't have one laying aroung, you can find these for next to nothing at the dollar store. Also, I had the blue ice laying around, but I can't see any reason why you couldn't just use a ziplock bag of ice instead of the blue ice.

And for anyone interested, that's 12 vials (8 - 32ml, 4 - 200ml) of Wyeast 1056 waiting to be dumped onto an unsuspecting wort...

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Old 08-28-2007, 05:20 PM   #8
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Another yeast freezing update:

Of the 8 vials, one froze. I'm not sure why as I was careful to get all the glycerin, water, yeast well mixed prior to and during use. Hmmm... glycerin is very heavy and layers sharply when added (pre-mixing). Perhaps there was some invisible layering taking place post mix. Better add glycerin to individual tubes and mix in situ.

Also the yeast has started changing color. The side facing the exterior of the lunchbox (see previous pic) is now a darker color (like trub), while the side facing the interior of the lunchbox (an icepack) is still light beige. I'm thinking excessive cooling (5* vs. 32*) and/or defrost cycle? In any case, this may warrant an ice jacket on the outside and bottom of the lunchbox for protection.

Yeast tube pic #1
Yeast tube pic #2

The pics are of the same tube. At no time was it allowed to lay on it's side and layer in that fashion.

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Old 08-28-2007, 05:34 PM   #9
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Quote:
Originally Posted by BierMuncher
I did a Kolsch last weekend with a 3-month old bottle. Turned out fine.

I have total confidence in any yeast I have that is less than 6 months old. Haven't gone longer than 6 months yet so can't really say.
was that three months in a fridge, or frozen?


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Old 08-28-2007, 09:44 PM   #10
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Quote:
Originally Posted by brian williams
was that three months in a fridge, or frozen?


brian
Fridge.

I haven't had to go long enough to worry about freezing yet. Plus, I don't have any glycerin on hand.
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