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Old 02-15-2008, 11:49 AM   #1
Schlenkerla
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Default Yeast Culturer's: Re-Using Slants?

When you take a few loops from a slant and now its considered spent, what do you do with them?

(Assumes they are not disposable or plastic test tubes.)

Do you:

1) Clean them out and refill then with media.
or
2) Just pressure cook them again, slant cool and re-noc.
Thanks!!
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Old 02-15-2008, 01:19 PM   #2
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When using gelatin, all you have to do is run the vial under hot tap water for a few seconds and the gelatin will melt and slide out. I do that, then just refill when I've got enough empties.

I suppose in theory you could just re-cook them, killing whatever yeast is left, as any yeast in the media would only serve as food to the new yeast anyway, but I have not tried it.

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Old 02-15-2008, 02:10 PM   #3
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Out of all the documentation I have read about this, I never see this explained.

Thanks Funkenjaeger!!!

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