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Old 08-07-2007, 01:06 PM   #1
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Default Washing yeast, starters, pre-made wort, all kinds of yeast Q's

I've got more questions that I've been trying to find here, but so far no good. I've been using my stir plate (which is beast, thanks for all of those endorsers of it) and I took some of my Wyeast 1332 that I have for a IPA I'm making any day now. Now I made some wort (pint of water, half cup of DME) and now have added 3 rounds of it, before the third I filled two 12 ounce bottles and stuck them in the fridge. By this point I am ecstatic! Not only did I make a starter, but I even have 2 extra bottles of yeast to make more IPA goodness later. So on to question number 1:

Why in my 1332 Wyeast, is there a sweet (and I mean sweet) layer of light colored (the usual) yeast in the bottom, with a smaller darker layer on top? Any ideas?

Next on my list is the topic of washing yeast. I washed my Hefe yeast, after a vigorous fermentation, and I got very little yeast. I expected so much, and I got so little. I was actually surprised but are there any tips to get more yeast out of a washing? I'll probably make more wort to propagate more of the Hefe yeast before I really store it...but I didn't get much

And for my last question, is there a easy way to make a huge batch of wort for starters up and freeze it or something? DME is so messy IMO, and I'd rather make a big batch, put them into freezer bags, and freeze.

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Old 08-07-2007, 01:35 PM   #2
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Quote:
Originally Posted by 98EXL
I've got more questions that I've been trying to find here, but so far no good. I've been using my stir plate (which is beast, thanks for all of those endorsers of it) and I took some of my Wyeast 1332 that I have for a IPA I'm making any day now. Now I made some wort (pint of water, half cup of DME) and now have added 3 rounds of it, before the third I filled two 12 ounce bottles and stuck them in the fridge. By this point I am ecstatic! Not only did I make a starter, but I even have 2 extra bottles of yeast to make more IPA goodness later. So on to question number 1:

Why in my 1332 Wyeast, is there a sweet (and I mean sweet) layer of light colored (the usual) yeast in the bottom, with a smaller darker layer on top? Any ideas?

Next on my list is the topic of washing yeast. I washed my Hefe yeast, after a vigorous fermentation, and I got very little yeast. I expected so much, and I got so little. I was actually surprised but are there any tips to get more yeast out of a washing? I'll probably make more wort to propagate more of the Hefe yeast before I really store it...but I didn't get much

And for my last question, is there a easy way to make a huge batch of wort for starters up and freeze it or something? DME is so messy IMO, and I'd rather make a big batch, put them into freezer bags, and freeze.
I'll speak to the last one. I think you could freeze the dme-wort for starters if you wanted to...but I wouldn't count on unabated sanitation. You'd still have to boil the wort again once it was thawed, just to make sure it was sanitized.
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Old 08-07-2007, 01:41 PM   #3
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I'm not sure why you did not get much yeast. Maybe you waited to long before transfering to the next jar?
I washed my Hefe yeast (WLP300) and have a little more then 2 cups of yeast out of it.
With a Small starter I can get maybe 10 more brews just from that. With a big starter, maybe more.

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Old 08-07-2007, 02:22 PM   #4
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About the HW yeast...you MAY have tossed out more of it than trub.

HW yeast is not creamy white ike with other yeasts. It is dark.

If the thin layer on top gets to looking like lake scum then you have an infection in there. Toss it.

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Old 08-07-2007, 02:50 PM   #5
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Quote:
Originally Posted by homebrewer_99
About the HW yeast...you MAY have tossed out more of it than trub.

HW yeast is not creamy white ike with other yeasts. It is dark.

If the thin layer on top gets to looking like lake scum then you have an infection in there. Toss it.

wait, that is kinda a contradiction.....Hefe yeast is dark, but if it looks like lake scum it's infected? Now I'm confused
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Old 08-07-2007, 04:52 PM   #6
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wait, that is kinda a contradiction.....Hefe yeast is dark, but if it looks like lake scum it's infected? Now I'm confused
No, I'm talking about a layer on top of the yeast that, it's kind of difficult to explain..., there's ANOTHER loose darker layer on top of the yeast. It reminds me of plant-life coming off the bottom of a lake.
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Old 08-07-2007, 04:53 PM   #7
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maybe I will take a nice macro shot of it later tonight for you

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Old 08-07-2007, 05:11 PM   #8
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I was thinking I wish I had taken pics of my samples for the same reason...

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Old 08-07-2007, 05:24 PM   #9
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well, I will take some pics tonight....I'm worried about the sanitation now.....but I'm so good about that. The other thing that I forgot to ask is I had vodka suckback into my starter...is that bad?

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Old 08-07-2007, 05:25 PM   #10
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Since when is vodka bad???

You're fine...

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