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Old 06-06-2009, 01:00 AM   #1
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Default Isolating colonies from streak plates

Bought some agar-agar from the local asian market, and isolated 3 colonies from each. Just wanted to share the pics. By the way, if you are forced to buy the "stick" instead of powdered agar, use about a half stick for each 300 mL of wort.






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Old 06-06-2009, 01:18 AM   #2
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Nice! I haven't gotten that advanced with my yeast (yet?), but it looks like you've got it down.

BTW, I first read the topic title as, "Isolating chocolate from steak plates."

Must get more sleep.

-Steve



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Old 06-06-2009, 01:25 AM   #3
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Have you used the powdered Agar-Agar (such as the Telephone brand)? I used that for my last set of slants and may have went a little stiff on the agar mix. They set up pretty solid. I used 12-13 gr per 500 mL and I think I could have got by with 5-6 gr. They will probably still work, though. Yeast seem to be less picky then I am....

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Old 06-06-2009, 01:29 AM   #4
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Yeah, half a stick is about 4 grams. So 5-6 grams for 500 mL is about exactly the rate I used.

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Old 06-06-2009, 01:30 AM   #5
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Quote:
Originally Posted by physfarm View Post
Have you used the powdered Agar-Agar (such as the Telephone brand)? I used that for my last set of slants and may have went a little stiff on the agar mix. They set up pretty solid. I used 12-13 gr per 500 mL and I think I could have got by with 5-6 gr. They will probably still work, though. Yeast seem to be less picky then I am....
That sounds about right to me. I use agar that I get from lab, and I typically go with 2% agar.

I should probably start making some plates myself, they've been subcultured so many times that I should get some individual colonies.


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