Guide to Making a Frozen Yeast Bank - Page 6

**Philip1993** · 11-09-2007, 03:02 PM

Quote:

Originally Posted by MadWeezel

One may also look at getting a separatory Funnel with stopcock, After making your starter and removing from the stirplate, transfer your slurry into the stopcock, seal let settle then sterilize the stopcock tip before pouring into the vials.

That was my thought too, but a 2L separatory funnel is big $$$.

I like the baby bottles! I'm using 35ml glass, and every so often one fails under the stress of freezing.

**jds** · 11-11-2007, 04:06 AM

Actually, after using a White Labs liquid yeast today, I'm pretty sure the soda bottle preforms are exactly what WL sells for their 'pitchable liquid yeast'.

mrkristofo · 11-12-2007, 06:21 AM

Quote:

Originally Posted by jds

Actually, after using a White Labs liquid yeast today, I'm pretty sure the soda bottle preforms are exactly what WL sells for their 'pitchable liquid yeast'.

My only concern with those would be sterilizing them. If you're making a yeast bank, you're going to be propagating from the cultures time after time. It would be prudent to go beyond simply sanitizing and fully sterilizing those suckers. It's not worth the risk of contamination, to me. I don't trust it if I can't autoclave it

98EXL · 11-13-2007, 01:41 PM

I do like those tubes....very very cool

**Philip1993** · 11-13-2007, 02:28 PM

Quote:

Originally Posted by mrkristofo

It would be prudent to go beyond simply sanitizing and fully sterilizing those suckers. It's not worth the risk of contamination, to me. I don't trust it if I can't autoclave it

That's certainly the most conservative method, but it raises the question; Do you actually autoclave your vials? I know that I certainly do not. I just drop them in boiling water for 5 min, cap, and cool.

mrkristofo · 11-14-2007, 03:29 AM

Quote:

Originally Posted by pldoolittle

That's certainly the most conservative method, but it raises the question; Do you actually autoclave your vials? I know that I certainly do not. I just drop them in boiling water for 5 min, cap, and cool.

Yes, I do in fact.

**Professor Frink** · 11-14-2007, 03:48 AM

When you work in a lab, you can get the vials in a sterile pack

mrkristofo · 11-14-2007, 04:49 AM

Quote:

Originally Posted by Professor Frink

When you work in a lab, you can get the vials in a sterile pack

This is also true. 15mL falcon tubes work great for saving a smidge of yeast in the fridge that's pitched into a starter. Get about 10 of those off a 1L culture.

**Philip1993** · 11-14-2007, 05:10 PM

Quote:

Originally Posted by mrkristofo

Yes, I do in fact.

Very cool. No easy access here. Anyway, I meant "you" in a broad sense. I wasn't trying to insinuate you specifically didn't, and I apologize if it read that way.

My (poorly delivered) point was that so often us techies tend to engineer things beyond what reality calls for. And for the majority of us who consider 'sterile' a 3 min dunk in a pot of boiling water, there's probably no perceivable benefit to paying extra for 'glassware' rated beyond 212*F

Now for you guys that do have access, light it up!

Bandit112 · 11-27-2007, 02:00 AM

nothing to say but....

thanks

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