One may also look at getting a separatory Funnel with stopcock, After making your starter and removing from the stirplate, transfer your slurry into the stopcock, seal let settle then sterilize the stopcock tip before pouring into the vials.
That was my thought too, but a 2L separatory funnel is big $$$.
I like the baby bottles! I'm using 35ml glass, and every so often one fails under the stress of freezing.
__________________
OdorDestroyer.com - Pet odor removers and related products.
Actually, after using a White Labs liquid yeast today, I'm pretty sure the soda bottle preforms are exactly what WL sells for their 'pitchable liquid yeast'.
Actually, after using a White Labs liquid yeast today, I'm pretty sure the soda bottle preforms are exactly what WL sells for their 'pitchable liquid yeast'.
My only concern with those would be sterilizing them. If you're making a yeast bank, you're going to be propagating from the cultures time after time. It would be prudent to go beyond simply sanitizing and fully sterilizing those suckers. It's not worth the risk of contamination, to me. I don't trust it if I can't autoclave it
__________________ Coming Soon: Primary: Stone Vertical Epic 08.08.08 Secondary: Oaked Arrogant Bastard Conditioning: Dead Guy Ale clone, Double-Dubbel Drinking: Not for a while
It would be prudent to go beyond simply sanitizing and fully sterilizing those suckers. It's not worth the risk of contamination, to me. I don't trust it if I can't autoclave it
That's certainly the most conservative method, but it raises the question; Do you actually autoclave your vials? I know that I certainly do not. I just drop them in boiling water for 5 min, cap, and cool.
__________________
OdorDestroyer.com - Pet odor removers and related products.
That's certainly the most conservative method, but it raises the question; Do you actually autoclave your vials? I know that I certainly do not. I just drop them in boiling water for 5 min, cap, and cool.
Yes, I do in fact.
__________________ Coming Soon: Primary: Stone Vertical Epic 08.08.08 Secondary: Oaked Arrogant Bastard Conditioning: Dead Guy Ale clone, Double-Dubbel Drinking: Not for a while
When you work in a lab, you can get the vials in a sterile pack
This is also true. 15mL falcon tubes work great for saving a smidge of yeast in the fridge that's pitched into a starter. Get about 10 of those off a 1L culture.
__________________ Coming Soon: Primary: Stone Vertical Epic 08.08.08 Secondary: Oaked Arrogant Bastard Conditioning: Dead Guy Ale clone, Double-Dubbel Drinking: Not for a while
Very cool. No easy access here. Anyway, I meant "you" in a broad sense. I wasn't trying to insinuate you specifically didn't, and I apologize if it read that way.
My (poorly delivered) point was that so often us techies tend to engineer things beyond what reality calls for. And for the majority of us who consider 'sterile' a 3 min dunk in a pot of boiling water, there's probably no perceivable benefit to paying extra for 'glassware' rated beyond 212*F
Now for you guys that do have access, light it up!
__________________
OdorDestroyer.com - Pet odor removers and related products.
Last edited by Philip1993; 11-14-2007 at 06:15 PM.
Linear Mode
