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Old 09-20-2011, 09:10 PM   #581
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Originally Posted by hopfen
So I found out that While Labs vials are 35mL

How much yeast do I need to put in them to allow me to make a 2 liter starter?

thanks,

hopfen
Like I said, there are a lot of assumptions going into any number, but (based on my playings with a hemocytometer) 100mL sounds about right to me based on good quality, average storage times.


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Old 09-20-2011, 10:37 PM   #582
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How is 100mL going to fit in a 35mL vial?



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Old 09-20-2011, 11:05 PM   #583
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How is 100mL going to fit in a 35mL vial?
It's not going to. Your frozen sample is going to have low viability, and it will be half-glycerin. You're simply going to need more volume of the frozen stuff than of the fresh stuff.
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Old 09-21-2011, 02:02 AM   #584
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Quote:
Originally Posted by hopfen
How is 100mL going to fit in a 35mL vial?
White Labs uses a process that highly concentrates the yeast to a much higher density than typical slurry.
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Old 09-21-2011, 01:11 PM   #585
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Thanks,

That's the part I was missing.

So if I have yeast that isn't frozen, (been in fridge in a mason jar from last week's brew), I can take 100mL and make a new 2 liter starter?

How long will it be ok in the fridge before I should freeze it?

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Old 09-21-2011, 01:18 PM   #586
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Thanks,

That's the part I was missing.

So if I have yeast that isn't frozen, (been in fridge in a mason jar from last week's brew), I can take 100mL and make a new 2 liter starter?

How long will it be ok in the fridge before I should freeze it?
If it hasn't been frozen, then you want to use something like the Mr. Malty pitching rate calculator to figure out how much slurry you need. That's largely a separate issue.

48h in the fridge before freezing is fairly standard.
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Old 09-21-2011, 04:11 PM   #587
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I'm using Mr Malty and now all of the pieces are fitting together.

So, if I don't have enough slurry for the batch that Mr Malty says I need, it sounds like I can take 100mL of slurry and make a new 2 liter starter, correct?

Wow, 48 hours - I thought you could go 2 weeks after washing yeast and storing it in the fridge.

thanks for all of the info.

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Old 09-21-2011, 04:28 PM   #588
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Quote:
Originally Posted by hopfen View Post
I'm using Mr Malty and now all of the pieces are fitting together.

So, if I don't have enough slurry for the batch that Mr Malty says I need, it sounds like I can take 100mL of slurry and make a new 2 liter starter, correct?

Wow, 48 hours - I thought you could go 2 weeks after washing yeast and storing it in the fridge.

thanks for all of the info.
Yep. You can make a starter out of slurry. In fact, it's a good idea.

Re: time in fridge. I misunderstood your question before. Many people stick their samples in the fridge for 48 hours before transferring to the freezer to let the yeast build up trehalose reserves. I now understand that you were asking about the maximum possible time. Slurry, if properly stored, can stay in the fridge for a long time. Months even. But, if you generally want very, very healthy yeast going into the freezer, since the long storage process is generally pretty hard on the cells. Generally, I would build a starter up and then freeze that slurry if I had a stock that had been sitting around for a couple of weeks.
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Old 11-16-2011, 05:25 PM   #589
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this is probably a stupid question, however i still feel inclined to ask. would it be possible to store the yeast sample in pre-sterilized transfer pipettes, and heat seal the end? I've any disadvantages?

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Old 12-12-2011, 05:20 AM   #590
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Greetings frozen yeast ranchers. I will be starting my own bank very shortly. I have all the equipment but I was waiting on a few items before I really got into this.

I will be using the cynmar 16ml glass vials that are mentioned. A thing of note to anyone using these vials, I have a semi cheap way to hold them individually without a PITA DIY. I believe and will confirm in a few days, that a 12/10 gauge shotgun shell case will hold 25 vials with no issue. I thought a 16/12 gauge would work but it is just a bit much to get them in and then back out. So I will use those for their intended purpose...the 12/10's are on the way.

My plan is to use the case to hold 10, gen 1 vials with 1 strain stretched between 2 boxes. I ordered 4 boxes from Midway, the shipping was a bit nasty but I require a good way to store these in my freezer, so I can currently have 10 strains in my bank.

What is the longest time someone has frozen and then revived a specimen?



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