Guide to Making a Frozen Yeast Bank

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So at the risk of a major thread necro, 4 years, 2 months later I appear to have brought back some Conan I froze in February 2016 using this guide. It survived a divorce and 2 moves. I used 4 soda bottle preform tubes from my “bank” inside a cooler bag with additional ice packs in my various freezers over time. The starter is on a stir plate producing significant CO2 after 3 days. Probably a longer lag time than usual, but if I remember correctly Conan always had a longer lag time.

Sounds good. I have a bunch of yeast that has been frozen longer than that. My longest resurrection so far is from a yeast that was frozen for a little over 6 years.
 
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