Happy HolidaySs Giveaway - Winners Re-Re-Re-Re-Drawn - 24 hours to Claim!

Get your HBT Growlers, Shirts and Membership before the Rush!


Home Brew Forums > Home Brewing Beer > General Techniques > Guide to Making a Frozen Yeast Bank
Reply
 
LinkBack Thread Tools
Old 10-15-2007, 03:57 AM   #41
Philip1993
Feedback Score: 0 reviews
Recipes 
 
Join Date: Jul 2007
Posts: 1,658
Liked 8 Times on 8 Posts
Likes Given: 4

Default

Quote:
Originally Posted by jdoiv
Ok, so I went by the LHBS this morning and picked up what I hope is the right stuff. If was Glycerin USP Finishing Formula from JD Carlson. Is this the right stuff?

Yep. padding
__________________
Philip1993 is offline
 
Reply With Quote Quick reply to this message
Old 10-15-2007, 03:17 PM   #42
camiller
Feedback Score: 0 reviews
Recipes 
 
Join Date: Apr 2007
Location: Omaha, NE
Posts: 1,883
Liked 29 Times on 29 Posts
Likes Given: 9

Default

Quote:
Originally Posted by jdoiv
If was Glycerin USP Finishing Formula from JD Carlson. ...

I Think that is LD Carlson. Since they sell it as an additive for wines and liqueurs it certainly should be safe for consumption.
__________________
camiller is offline
 
Reply With Quote Quick reply to this message
Old 10-15-2007, 11:08 PM   #43
PintOfBitter
Sponsor
HBT_SPONSOR.png
Vendor Ads 
Feedback Score: 0 reviews
 
PintOfBitter's Avatar
Recipes 
 
Join Date: Feb 2007
Location: USA
Posts: 1,175
Liked 9 Times on 8 Posts
Likes Given: 3

Default

Wow, this sounds like something I'd like to try. I don't have any of the glassware needed, but I was looking around on that Cynmar company's site, and it looks like the whole "kit" could be pieced together pretty cheap. Maybe I'll go for it.

For how many generations is a given yeast supposed to be good for? I'm sure this process shouldn't be repeated forever, right? It seems like I remember the magic number of 4 generations from somewhere...

__________________

-PoB

Maybe you can use these Grain, Hops, Yeast Reference Charts
Come visit WildHopsPrintShop.com- custom bottle caps, beer glassware, tap handles, and more!

PintOfBitter is offline
 
Reply With Quote Quick reply to this message
Old 10-16-2007, 02:13 AM   #44
left field brewer
Feedback Score: 0 reviews
Recipes 
 
Join Date: Jul 2007
Location: Lansing, MI
Posts: 94
Liked 1 Times on 1 Posts

Default

Thanks for the info. I'm just starting to get into yeast banking. Is that a Corning PC-353? I got the same one at the university surplus store for $5. Its already paid for itself after making just a couple of starters, and the amount of yeast it produces per volume is pretty sweet. Also a little off topic but how hard was it to tap a valve into the top of the pressure cooker(pretty thick)? I'd hate to ruin the nice new $90 pressure cooker I'm looking at.

__________________
left field brewer is offline
 
Reply With Quote Quick reply to this message
Old 10-16-2007, 02:16 AM   #45
Philip1993
Feedback Score: 0 reviews
Recipes 
 
Join Date: Jul 2007
Posts: 1,658
Liked 8 Times on 8 Posts
Likes Given: 4

Default

Quote:
Originally Posted by PintOfBitter
Wow, this sounds like something I'd like to try. I don't have any of the glassware needed, but I was looking around on that Cynmar company's site, and it looks like the whole "kit" could be pieced together pretty cheap. Maybe I'll go for it.
Scientific glassware is nice, but you can get everything you need at the grocery, just remove the food and wash. Of all the pieces I have, the two I like best came filled with apple juice and Ragu...
__________________
Philip1993 is offline
mavtitle Likes This 
Reply With Quote Quick reply to this message
Old 10-17-2007, 04:10 AM   #46
FlyGuy
Feedback Score: 0 reviews
 
FlyGuy's Avatar
Recipes 
 
Join Date: Jan 2007
Location: Calgary, Alberta
Posts: 3,618
Liked 136 Times on 42 Posts
Likes Given: 7

Default

Quote:
Originally Posted by left field brewer
Thanks for the info. I'm just starting to get into yeast banking. Is that a Corning PC-353? I got the same one at the university surplus store for $5. Its already paid for itself after making just a couple of starters, and the amount of yeast it produces per volume is pretty sweet. Also a little off topic but how hard was it to tap a valve into the top of the pressure cooker(pretty thick)? I'd hate to ruin the nice new $90 pressure cooker I'm looking at.
Yep, I got mine surplus at my university, too! Works great still.

Regarding tapping the valve, it was dead easy. But I would recommend to anyone to be very cautious when you do it. If it isn't threaded properly, you are just asking for serious trouble. To anyone buying a new pressure cooker, I would HIGHLY recommend finding one that has a pressure relief valve that unscrews -- then you can add a tee fitting, put the pressure relief valve back on, and then add your own needle valve for the steam outlet. Brewman ! has pics of his system (see top of post for links).
FlyGuy is offline
 
Reply With Quote Quick reply to this message
Old 10-17-2007, 09:01 PM   #47
MadWeezel
Feedback Score: 0 reviews
Recipes 
 
Join Date: Jun 2006
Location: Clive, Ia
Posts: 113
Default

One may also look at getting a separatory Funnel with stopcock, After making your starter and removing from the stirplate, transfer your slurry into the stopcock, seal let settle then sterilize the stopcock tip before pouring into the vials.


1000ml

250ml

__________________
MadWeezel is offline
 
Reply With Quote Quick reply to this message
Old 10-26-2007, 04:30 PM   #48
Andy77
Feedback Score: 0 reviews
Recipes 
 
Join Date: Oct 2007
Posts: 17
Default

Just FYI, you can get anything you need from a scientific supply house like Fisher Scientific or VWR, I plan on making my frozen stock soon, you can get sterile tubes, anything you need.

__________________
Andy77 is offline
 
Reply With Quote Quick reply to this message
Old 10-26-2007, 04:36 PM   #49
mrkristofo
Feedback Score: 0 reviews
 
mrkristofo's Avatar
Recipes 
 
Join Date: Sep 2007
Location: Behind the zion curtain
Posts: 941
Liked 6 Times on 6 Posts

Default

Quote:
Originally Posted by MadWeezel
One may also look at getting a separatory Funnel with stopcock, After making your starter and removing from the stirplate, transfer your slurry into the stopcock, seal let settle then sterilize the stopcock tip before pouring into the vials.


1000ml

250ml

I can't believe I didn't think of this. Well done. Really, good form.
__________________
Coming Soon:
Primary: Stone Vertical Epic 08.08.08
Secondary: Oaked Arrogant Bastard
Conditioning: Dead Guy Ale clone, Double-Dubbel
Drinking: Not for a while
mrkristofo is offline
 
Reply With Quote Quick reply to this message
Old 11-09-2007, 02:34 PM   #50
jds
Feedback Score: 0 reviews
 
jds's Avatar
Recipes 
 
Join Date: Oct 2007
Location: Littleton, CO
Posts: 1,947
Liked 18 Times on 15 Posts

Default

Quick question / addition:

I've seen these baby soda bottles online for a fairly low price. They're preforms for 2 l soda bottles.



It's a bit hard to tell from the photos, but they look to be about the size of the White Labs pitchable liquid yeasts. I'm planning on saving and freezing some yeast from my next batch. Anyhow, I'm thinking these might be a good size for both storing/freezing yeast, and possibly building a mini-starter in as well.

Has anybody used these for yeast storage or starters?

__________________
jds is offline
 
Reply With Quote Quick reply to this message
Reply



Quick Reply
Message:
Options
Thread Tools


Similar Threads
Thread Thread Starter Forum Replies Last Post
Frozen Yeast Bank vs. Yeast Slanting shortyjacobs Fermentation & Yeast 11 09-24-2010 08:13 PM
Frozen yeast bank not freezing T-Hops General Techniques 6 03-17-2010 04:20 PM
Yeast Starter from frozen yeast bank dfohio General Techniques 5 04-30-2009 08:32 PM
Frozen Yeast Bank- Strain Exchange? flyangler18 General Techniques 37 03-25-2009 02:17 PM
Frozen yeast bank with yeast cakes? mrfocus Recipes/Ingredients 5 11-08-2007 02:13 AM



Newest Threads

LATEST SPONSOR DEALS