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Guide to Making a Frozen Yeast Bank Guide to Making a Frozen Yeast Bank
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Old 01-21-2010, 10:37 AM   #251
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FlyGuy, great post! I have one question. I don't have the freezer space at this point. Instead of any cooler, do you think wrapping the refreezable ice bags around vials and wrap with a bugi cord will work?
I don't think it would work as well as using a cooler, but I bet it would work. You just might not see the same length of yeast viability.
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Old 01-21-2010, 02:52 PM   #252
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Originally Posted by Stuntmantoo View Post
FlyGuy, great post! I have one question. I don't have the freezer space at this point. Instead of any cooler, do you think wrapping the refreezable ice bags around vials and wrap with a bugi cord will work?
I did exactly this for a while. It will work, just make sure you get completely around the vials, including the top and bottom.
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Old 01-21-2010, 05:08 PM   #253
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I don't think it would work as well as using a cooler, but I bet it would work. You just might not see the same length of yeast viability.
Appreciate it much, you too Scimmia
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Old 01-21-2010, 09:09 PM   #254
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Originally Posted by Stuntmantoo View Post
FlyGuy, great post! I have one question. I don't have the freezer space at this point. Instead of any cooler, do you think wrapping the refreezable ice bags around vials and wrap with a bugi cord will work?
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Originally Posted by FlyGuy View Post
I don't think it would work as well as using a cooler, but I bet it would work. You just might not see the same length of yeast viability.
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Originally Posted by Scimmia View Post
I did exactly this for a while. It will work, just make sure you get completely around the vials, including the top and bottom.
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Originally Posted by Stuntmantoo View Post
Appreciate it much, you too Scimmia
And I'd put the whole thing inside a ziplock, the bag will keep interaction with the air in the freezer during the defrost cycle to a minimum.
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Old 01-21-2010, 10:59 PM   #255
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How cold can you freeze yeast without damaging them without taking special precautions?
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Old 01-22-2010, 12:14 AM   #256
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How cold can you freeze yeast without damaging them without taking special precautions?
None. The transition to a frozen state is what damages them.

Once frozen, they stay preserved best at the coldest temperatures (e.g., labs store yeast at around -80).
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Old 01-22-2010, 12:21 AM   #257
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None. The transition to a frozen state is what damages them.

Once frozen, they stay preserved best at the coldest temperatures (e.g., labs store yeast at around -80).
Are you saying this is at 0C?
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Old 01-22-2010, 01:38 AM   #258
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Are you saying this is at 0C?
Actually, I believe the most damage occurs just below that (by a few degrees). That is why allowing the yeast to freeze then thaw then freeze then thaw (e.g., with a frost-free freezer) you can really do them harm.
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Old 01-28-2010, 02:34 AM   #259
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Hey FlyGuy,

What type of pipette do you use? I have everything except the yeast collector, I was thinking of getting a graduated glass pipette and bulb. The one I'm looking at (10mL) is about 14" long. Tough to fit in the pressure cooker.

Other than the turkey baster, what are people using for this?
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Old 01-28-2010, 11:23 AM   #260
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I am using a graduated glass pipette and a rubber bulb now. I have a big 5.5 gal pressure cooker now, and the pipette fits inside.

The turkey baster actually worked OK though.
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