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Old 05-11-2008, 09:34 AM   #131
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...And 0.0017% is the mathematical equivalent of RDWHAHB...
Haha that's awesome, signaturised.
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Old 05-16-2008, 12:42 AM   #132
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frozen yeast bank stuff...thought it was pretty interesting(sold in a kit)
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Old 05-16-2008, 09:18 AM   #133
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OK not exactly specific to this topic but don't think it's worth starting a new thread for.

I made my second starter with a tube of WLP028 to freeze the yeast as outlined.

It was a 2L starter, ~1.030, on a stirplate for 48 hours, cooled in the kegerator to approx 7degC for 18 hours and it fell pretty clear. I tasted a little and it tasted like fermented beer. Poured off all but about 30mL of beer, and swirled the remaining up to suspend the pretty thick sediment. I managed to fill about 10 tubes with 10mL of medium-thin slurry so about 100mL of slurry was gained from the 2L starter. But judging from...

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Originally Posted by FlyGuy View Post
...You should still have plenty of yeast left in the flask to pitch into a batch of beer...
Does what I got (100mL/3.4oz slurry including hot break) sound about right? Seems like a small amount of yeast from such a large starter. Was 48 hours too short? Did I bugger up somewhere?

Cheers.
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Old 05-16-2008, 01:53 PM   #134
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After decanting the beer and adding back distilled water, I harvest about 180ml of slurry from a 1L starter and that settles out into about 60-65ml of compacted yeast.

1.030 is a little low (I use .040) and I would have stepped up the starter from 500ml to 2L in 500ml increments. Also, be sure to use a foam plug or foil instead of an airlock to encourage air exchange.
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Last edited by Philip1993; 05-16-2008 at 02:00 PM.
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Old 05-16-2008, 01:56 PM   #135
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Originally Posted by pldoolittle View Post
1.030 is a little low (I use .040) and I would have stepped up the starter from 500ml to 2L in 500ml increments. Also, be sure to use a foam plug or foil instead of an airlock to encourage air exchange.
I use a stirplate, should I not be using an airlock?
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Old 05-16-2008, 02:31 PM   #136
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I use a stirplate, should I not be using an airlock?
There are lot of people that do, but presently CW says no. Airlock don't allow air into the vessel.
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Old 05-16-2008, 02:48 PM   #137
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Quote:
Originally Posted by pldoolittle View Post
There are lot of people that do, but presently CW says no. Airlock don't allow air into the vessel.
Wow, cool...So tinfoil is permeable enough?
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Old 05-16-2008, 03:04 PM   #138
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The foil is not permeable, but it's just draped loosely over the top as a dust cover and crimped a bit so it doesn't fall/blow off. Look at this pic from the beginning of the thread.

http://www.homebrewtalk.com/351734-post2.html
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Old 05-16-2008, 08:35 PM   #139
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Quote:
Originally Posted by pldoolittle View Post
After decanting the beer and adding back distilled water, I harvest about 180ml of slurry from a 1L starter and that settles out into about 60-65ml of compacted yeast.

1.030 is a little low (I use .040) and I would have stepped up the starter from 500ml to 2L in 500ml increments. Also, be sure to use a foam plug or foil instead of an airlock to encourage air exchange.
Thanks, I was using foil over the top of the flask.

I started with a 'pitchable' vial from White Labs, is stepping up still neccessary?

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Old 05-17-2008, 04:16 AM   #140
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I started with a 'pitchable' vial from White Labs, is stepping up still neccessary?
No, but it seems to me that you end up with more yeast by stepping up than starting with a full wort.
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