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Old 05-11-2008, 08:34 AM   #131
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Originally Posted by pldoolittle View Post
...And 0.0017% is the mathematical equivalent of RDWHAHB...
Haha that's awesome, signaturised.
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Old 05-15-2008, 11:42 PM   #132
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frozen yeast bank stuff...thought it was pretty interesting(sold in a kit)
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Old 05-16-2008, 08:18 AM   #133
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OK not exactly specific to this topic but don't think it's worth starting a new thread for.

I made my second starter with a tube of WLP028 to freeze the yeast as outlined.

It was a 2L starter, ~1.030, on a stirplate for 48 hours, cooled in the kegerator to approx 7degC for 18 hours and it fell pretty clear. I tasted a little and it tasted like fermented beer. Poured off all but about 30mL of beer, and swirled the remaining up to suspend the pretty thick sediment. I managed to fill about 10 tubes with 10mL of medium-thin slurry so about 100mL of slurry was gained from the 2L starter. But judging from...

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...You should still have plenty of yeast left in the flask to pitch into a batch of beer...
Does what I got (100mL/3.4oz slurry including hot break) sound about right? Seems like a small amount of yeast from such a large starter. Was 48 hours too short? Did I bugger up somewhere?

Cheers.
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Old 05-16-2008, 12:53 PM   #134
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After decanting the beer and adding back distilled water, I harvest about 180ml of slurry from a 1L starter and that settles out into about 60-65ml of compacted yeast.

1.030 is a little low (I use .040) and I would have stepped up the starter from 500ml to 2L in 500ml increments. Also, be sure to use a foam plug or foil instead of an airlock to encourage air exchange.

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Old 05-16-2008, 12:56 PM   #135
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1.030 is a little low (I use .040) and I would have stepped up the starter from 500ml to 2L in 500ml increments. Also, be sure to use a foam plug or foil instead of an airlock to encourage air exchange.
I use a stirplate, should I not be using an airlock?
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Old 05-16-2008, 01:31 PM   #136
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I use a stirplate, should I not be using an airlock?
There are lot of people that do, but presently CW says no. Airlock don't allow air into the vessel.
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Old 05-16-2008, 01:48 PM   #137
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There are lot of people that do, but presently CW says no. Airlock don't allow air into the vessel.
Wow, cool...So tinfoil is permeable enough?
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Old 05-16-2008, 02:04 PM   #138
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The foil is not permeable, but it's just draped loosely over the top as a dust cover and crimped a bit so it doesn't fall/blow off. Look at this pic from the beginning of the thread.

http://www.homebrewtalk.com/showpost...34&postcount=2

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Old 05-16-2008, 07:35 PM   #139
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Quote:
Originally Posted by pldoolittle View Post
After decanting the beer and adding back distilled water, I harvest about 180ml of slurry from a 1L starter and that settles out into about 60-65ml of compacted yeast.

1.030 is a little low (I use .040) and I would have stepped up the starter from 500ml to 2L in 500ml increments. Also, be sure to use a foam plug or foil instead of an airlock to encourage air exchange.
Thanks, I was using foil over the top of the flask.

I started with a 'pitchable' vial from White Labs, is stepping up still neccessary?

Cheers.
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Old 05-17-2008, 03:16 AM   #140
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Originally Posted by denimglen View Post
I started with a 'pitchable' vial from White Labs, is stepping up still neccessary?
No, but it seems to me that you end up with more yeast by stepping up than starting with a full wort.
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