Guide to Making a Frozen Yeast Bank - Page 12

scinerd3000 · 05-02-2008, 07:03 AM

so when you guys are all talking about slants- what type of agar are you using? also im wondering how you stage those since the yeast cells will still proliferate on the slants even in the fridge...

scinerd3000 · 05-02-2008, 07:08 AM

found this one...do you think this would work? or am i better off using the glycerin freeze method?

http://www.sigmaaldrich.com/img/assets/6840/70196_Wort_Agar.pdf

Philip1993 · 05-02-2008, 02:13 PM

Most of us aren't using slants because of the volume of yeast produced in a wort, and the extra work involved in propagation. I use glycerin and store 32ml samples ready to pitch into a starter. They look like this:

scinerd3000 · 05-02-2008, 06:43 PM

Quote:

Originally Posted by pldoolittle

Most of us aren't using slants because of the volume of yeast produced in a wort, and the extra work involved in propagation. I use glycerin and store 32ml samples ready to pitch into a starter. They look like this:

what ratio of glycerin/water/yeast do you use? and have you had any viability issues? IM seriously looking into this because yeast is becoming the most expensive part of my brewing now that i planted hops. I wonder if anyone grows and malts their own grain?

also where did you get the 32ml tubes from?

mrkristofo · 05-03-2008, 04:12 AM

Quote:

Originally Posted by scinerd3000

what ratio of glycerin/water/yeast do you use? and have you had any viability issues? IM seriously looking into this because yeast is becoming the most expensive part of my brewing now that i planted hops. I wonder if anyone grows and malts their own grain?

also where did you get the 32ml tubes from?

Take a sample of suspended yeast (in wort/beer/etc) and mix it so that the final concentration is 15-20% gylcerol (glycerine).

Professor Frink · 05-03-2008, 04:33 AM

Quote:

Originally Posted by scinerd3000

found this one...do you think this would work? or am i better off using the glycerin freeze method?

http://www.sigmaaldrich.com/img/assets/6840/70196_Wort_Agar.pdf

That would work for slants. Sigma-aldrich - that could go on an NIH grant...

Professor Frink · 05-03-2008, 04:35 AM

Quote:

Originally Posted by scinerd3000

what ratio of glycerin/water/yeast do you use? and have you had any viability issues? IM seriously looking into this because yeast is becoming the most expensive part of my brewing now that i planted hops. I wonder if anyone grows and malts their own grain?

also where did you get the 32ml tubes from?

I've grown up at least a half dozen frozen tubes, and never had viability issues. Some have taken a little while to get going, but they all worked fine.

Philip1993 · 05-03-2008, 04:40 AM

Quote:

Originally Posted by scinerd3000

what ratio of glycerin/water/yeast do you use? and have you had any viability issues? also where did you get the 32ml tubes from?

20% glycerin to slurry (~6-7ml/tube). No issues. I found the tubes on ebay for about 50c each..

**wedge421** · 05-06-2008, 03:33 AM

So I picked up a bottle of glycerine from the Rx today. It says for external use only though. In this diluted form that you end up making is it ok to use? Or do I need to get some type of food grade glycerine?

Philip1993 · 05-06-2008, 04:23 AM

Quote:

Originally Posted by wedge421

So I picked up a bottle of glycerine from the Rx today. It says for external use only though. In this diluted form that you end up making is it ok to use? Or do I need to get some type of food grade glycerine?

As long as it's just glycerin and not glycerin with {rose hips, aloe, XXXX}, it's fine.