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Old 05-02-2008, 06:03 AM   #111
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so when you guys are all talking about slants- what type of agar are you using? also im wondering how you stage those since the yeast cells will still proliferate on the slants even in the fridge...

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Old 05-02-2008, 06:08 AM   #112
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found this one...do you think this would work? or am i better off using the glycerin freeze method?

http://www.sigmaaldrich.com/img/assets/6840/70196_Wort_Agar.pdf

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Old 05-02-2008, 01:13 PM   #113
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Most of us aren't using slants because of the volume of yeast produced in a wort, and the extra work involved in propagation. I use glycerin and store 32ml samples ready to pitch into a starter. They look like this:

the_yeasties.jpg  
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Old 05-02-2008, 05:43 PM   #114
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Quote:
Originally Posted by pldoolittle
Most of us aren't using slants because of the volume of yeast produced in a wort, and the extra work involved in propagation. I use glycerin and store 32ml samples ready to pitch into a starter. They look like this:
what ratio of glycerin/water/yeast do you use? and have you had any viability issues? IM seriously looking into this because yeast is becoming the most expensive part of my brewing now that i planted hops. I wonder if anyone grows and malts their own grain?

also where did you get the 32ml tubes from?
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Old 05-03-2008, 03:12 AM   #115
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Quote:
Originally Posted by scinerd3000
what ratio of glycerin/water/yeast do you use? and have you had any viability issues? IM seriously looking into this because yeast is becoming the most expensive part of my brewing now that i planted hops. I wonder if anyone grows and malts their own grain?

also where did you get the 32ml tubes from?
Take a sample of suspended yeast (in wort/beer/etc) and mix it so that the final concentration is 15-20% gylcerol (glycerine).
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Old 05-03-2008, 03:33 AM   #116
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Quote:
Originally Posted by scinerd3000
found this one...do you think this would work? or am i better off using the glycerin freeze method?

http://www.sigmaaldrich.com/img/assets/6840/70196_Wort_Agar.pdf
That would work for slants. Sigma-aldrich - that could go on an NIH grant...
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Old 05-03-2008, 03:35 AM   #117
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Quote:
Originally Posted by scinerd3000
what ratio of glycerin/water/yeast do you use? and have you had any viability issues? IM seriously looking into this because yeast is becoming the most expensive part of my brewing now that i planted hops. I wonder if anyone grows and malts their own grain?

also where did you get the 32ml tubes from?
I've grown up at least a half dozen frozen tubes, and never had viability issues. Some have taken a little while to get going, but they all worked fine.
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Old 05-03-2008, 03:40 AM   #118
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Quote:
Originally Posted by scinerd3000
what ratio of glycerin/water/yeast do you use? and have you had any viability issues? also where did you get the 32ml tubes from?
20% glycerin to slurry (~6-7ml/tube). No issues. I found the tubes on ebay for about 50c each..
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Old 05-06-2008, 02:33 AM   #119
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So I picked up a bottle of glycerine from the Rx today. It says for external use only though. In this diluted form that you end up making is it ok to use? Or do I need to get some type of food grade glycerine?

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Old 05-06-2008, 03:23 AM   #120
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Originally Posted by wedge421 View Post
So I picked up a bottle of glycerine from the Rx today. It says for external use only though. In this diluted form that you end up making is it ok to use? Or do I need to get some type of food grade glycerine?
As long as it's just glycerin and not glycerin with {rose hips, aloe, XXXX}, it's fine.
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