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02-25-2009, 04:40 PM
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#1
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Location: Hanover, PA
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Frozen Yeast Bank- Strain Exchange?
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I've been thinking about doing this for a while, especially as I have been banking more and more of the strains that I commonly keep on hand. Given the popularity of FlyGuy's frozen yeast bank tutorial, I'm guessing that there are a few HBTers keeping a bank of strains. Is there any interest in exchanging vials to expand our own yeast libraries?
Note your interest here, and I'll have a go at organizing some more formal.
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02-25-2009, 04:41 PM
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#2
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I've thought about the same thing. Although, I don't keep my bank in frozen storage, just in cold storage. Either way, a vial exchange would be great.
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"This is grain, which any fool can eat, but for which the Lord intended a more divine means of consumption. Let us give praise to our maker and glory to his bounty by learning about... BEER." - Friar Tuck
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02-25-2009, 04:46 PM
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#3
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Quote:
Originally Posted by tedski
I've thought about the same thing. Although, I don't keep my bank in frozen storage, just in cold storage. Either way, a vial exchange would be great.
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Ted,
Since you aren't freezing, are you slanting on agar or gelatin? I've not committed to one method vs. another just yet. |
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02-25-2009, 06:23 PM
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#4
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Quote:
Originally Posted by flyangler18
Ted,
Since you aren't freezing, are you slanting on agar or gelatin? I've not committed to one method vs. another just yet.
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I'm storing on slants made with 2% agar, 1.038 wort, and yeast nutrient.
I do 5ml of wort in 16ml culture vials.
__________________
"This is grain, which any fool can eat, but for which the Lord intended a more divine means of consumption. Let us give praise to our maker and glory to his bounty by learning about... BEER." - Friar Tuck
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02-25-2009, 08:04 PM
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#5
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Join Date: Dec 2008
Location: Austin, Texas
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I would love to exchange a few. I have Antwerp Ale (White Labs), Chimay's, Brooklyn Brewery's (local one), Rogue's PacMan, California Ale (White Labs), and am in process of aquiring White Labs British Ale strain. All are 4 ml of clean slurry (cells) in 15% glycerol and are stored at -4F.
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Give a man a beer and he will ask for more.
Teach a man to brew and you can ask him!
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02-25-2009, 08:05 PM
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#6
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Location: Birmingham, Al
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I am up for an exchange.
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Black Warrior Brewery
Primary's: Hob Goblin II, JOAM, Bleached Blonde Ale, Blonde Helles
Kegged: Imperial Honey Porter, JZ American Brown Ale, Scottish 80/-, Lake Walk Pale Ale, EdWort's Apfelwein, Parti-gyle Porter, Blueberry Blonde
Coming Up: Westvleteren 12 clone, Oktoberfast, SWMBO Slayer
Projects: Fermentation Chamber
Beer Blog
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02-25-2009, 08:22 PM
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#7
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Location: East Dundee, Illinois
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I am in for the exchange. I have Wyeast Irish Ale 1084 in frozen 20 ml glass vials. 15% glycerin kept in a cooler in the freezer.
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"People should not be afraid of their governments. Governments should be afraid of their people." - V
Primary: Nothin
Secondary: Shady Lord RIS, Water to Barleywine, Pumpkin wine, burnt mead
Kegged: Crappy infected mild
Bottles: Apfelwein, 999 Barleywine, Oatmeal Stout, Robust Porter, Robust smoked porter, Simcoe Smash
Last edited by conpewter; 02-26-2009 at 04:34 PM.
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02-26-2009, 02:37 PM
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#8
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Question for yeast-freezers: do any of you plate the culture after rewarming to room temp or does everyone pitch into a small starter like the tutorial shows?
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02-26-2009, 04:37 PM
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#9
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I pitch a 20 ml vial into 1 liter of wort on a stirplate
I write about it a little here
http://www.homebrewtalk.com/f13/aging-beer-facts-myths-discussion-84005/index3.html#post1082404
here is the article, and snipped text
Quote:
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Close examination of Figure 3 indicates that yeast should not be diluted more than 200 times the previous volume. In otherwords 10 ml should not be stepped up to more than 2 liters. The rationale behind this is simple. During yeast propagation it is important to keep the yeast growing exponentially (Figure 3, phase III). Diluting the yeast out too far will slow down their growth and give bacteria a chance to overtake the culture.
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MB Raines, Ph.D. - Guide to Yeast Culturing for Homebrewers - Maltose Falcons Home Brewing Society (Los Angeles Homebrewing)
__________________
"People should not be afraid of their governments. Governments should be afraid of their people." - V
Primary: Nothin
Secondary: Shady Lord RIS, Water to Barleywine, Pumpkin wine, burnt mead
Kegged: Crappy infected mild
Bottles: Apfelwein, 999 Barleywine, Oatmeal Stout, Robust Porter, Robust smoked porter, Simcoe Smash
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02-26-2009, 06:38 PM
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#10
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Join Date: Dec 2008
Location: Austin, Texas
Posts: 30
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I also pitch into a starter, 4ml cells into ~500ml lme, with a stirplate.
Great article from Dr. Raines. Thanks
__________________
Give a man a beer and he will ask for more.
Teach a man to brew and you can ask him!
Last edited by Hombru1; 02-26-2009 at 06:48 PM.
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