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-   -   Frozen Yeast Bank- Strain Exchange? (http://www.homebrewtalk.com/f13/frozen-yeast-bank-strain-exchange-105491/)

flyangler18 02-25-2009 04:40 PM

Frozen Yeast Bank- Strain Exchange?
 
I've been thinking about doing this for a while, especially as I have been banking more and more of the strains that I commonly keep on hand. Given the popularity of FlyGuy's frozen yeast bank tutorial, I'm guessing that there are a few HBTers keeping a bank of strains. Is there any interest in exchanging vials to expand our own yeast libraries?

Note your interest here, and I'll have a go at organizing some more formal.

tedski 02-25-2009 04:41 PM

I've thought about the same thing. Although, I don't keep my bank in frozen storage, just in cold storage. Either way, a vial exchange would be great.

flyangler18 02-25-2009 04:46 PM

Quote:

Originally Posted by tedski (Post 1158294)
I've thought about the same thing. Although, I don't keep my bank in frozen storage, just in cold storage. Either way, a vial exchange would be great.

Ted,

Since you aren't freezing, are you slanting on agar or gelatin? I've not committed to one method vs. another just yet.

tedski 02-25-2009 06:23 PM

Quote:

Originally Posted by flyangler18 (Post 1158308)
Ted,

Since you aren't freezing, are you slanting on agar or gelatin? I've not committed to one method vs. another just yet.

I'm storing on slants made with 2% agar, 1.038 wort, and yeast nutrient.

I do 5ml of wort in 16ml culture vials.

Hombru1 02-25-2009 08:04 PM

I would love to exchange a few. I have Antwerp Ale (White Labs), Chimay's, Brooklyn Brewery's (local one), Rogue's PacMan, California Ale (White Labs), and am in process of aquiring White Labs British Ale strain. All are 4 ml of clean slurry (cells) in 15% glycerol and are stored at -4F.

bashe 02-25-2009 08:05 PM

I am up for an exchange.

conpewter 02-25-2009 08:22 PM

I am in for the exchange. I have Wyeast Irish Ale 1084 in frozen 20 ml glass vials. 15% glycerin kept in a cooler in the freezer.

fratermus 02-26-2009 02:37 PM

Question for yeast-freezers: do any of you plate the culture after rewarming to room temp or does everyone pitch into a small starter like the tutorial shows?

conpewter 02-26-2009 04:37 PM

I pitch a 20 ml vial into 1 liter of wort on a stirplate

I write about it a little here
http://www.homebrewtalk.com/f13/agin...ml#post1082404

here is the article, and snipped text
Quote:

Close examination of Figure 3 indicates that yeast should not be diluted more than 200 times the previous volume. In otherwords 10 ml should not be stepped up to more than 2 liters. The rationale behind this is simple. During yeast propagation it is important to keep the yeast growing exponentially (Figure 3, phase III). Diluting the yeast out too far will slow down their growth and give bacteria a chance to overtake the culture.
MB Raines, Ph.D. - Guide to Yeast Culturing for Homebrewers - Maltose Falcons Home Brewing Society (Los Angeles Homebrewing)

Hombru1 02-26-2009 06:38 PM

I also pitch into a starter, 4ml cells into ~500ml lme, with a stirplate.

Great article from Dr. Raines. Thanks


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