It's best to take three separate samples of well-mixed and particle-free wort for refract readings. Protein globs and hop chunks can throw the reading off, as will sampling from the surface of a wort that has sat undisturbed for more than a minute or so. If you get three consistent readings, you are good to go.
I wouldn't worry though - your OG reading is pretty inconsequential unless something MAJOR went wrong. Even if you are ten points off, that's only going to affect your final gravity by 2 points or so.
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The Fiesty(sic) Goat Brewery est. 2007 & Clusterfuggle Experimental Ales est. 2009
Planned: Fat Man Brown Porter (Pro-Am #2), WLP 351 Hefeweizen, WLP 860 Munich Helles
Primary: Centennial Falcon IPA (Pro-Am #1), sLambic I
Secondary: Flanders Red
Kegged:Himmel un Ääd Kölsch #8, Farmhouse Session Saison Pilot Batch, Chocolate Milk Stout, Pale Ale, Chili Smoked Porter, Berliner Weisse w/ Brett #3
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