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-   -   Yeast biologists - worried about contaminating lab strains? (http://www.homebrewtalk.com/f128/yeast-biologists-worried-about-contaminating-lab-strains-310392/)

ScoRas 03-05-2012 04:33 PM

Yeast biologists - worried about contaminating lab strains?
 
Do any of you other yeast biologists/geneticists worry about contaminating your lab with wild/brewing strains of yeast. I know our lab strains generally have very large cell size, etc., but I don't want to mess up any experiments by bringing brewer's yeast into the lab.

I guess proper technique trumps all, yeast can come from anywhere!

j1laskey 03-05-2012 04:36 PM

I don't bring my brewing yeast into my TC lab.

ScoRas 03-05-2012 05:01 PM

Quote:

Originally Posted by j1laskey (Post 3859895)
I don't bring my brewing yeast into my TC lab.

Oh absolutely, contaminating mammalian cell lines would be a no, no. We even have a hard time convincing the flow cytometry core to run our live yeast surface-display samples.

I work on directed evolution in yeast, no mammalian cells in our lab. The PI is cool with it, another PI down the hall has a brewing yeast bank. My PI seems to agree, it's up to the individual researchers to maintain pure strains.

BeerDoctor5 03-06-2012 02:25 PM

You have nothing to worry about:D

I don't think bacteriologists worry about exogenous E.coli contaminating their labs.

Just don't create your starter on your work stir plate and you should be fine! My E.coli from my work that contains a plasmid with beta-amylase hasn't contaminated my brewing yet...but that might just increase my efficiency:p

ScoRas 03-06-2012 03:33 PM

Quote:

Originally Posted by BeerDoctor5 (Post 3863290)
You have nothing to worry about:D

I don't think bacteriologists worry about exogenous E.coli contaminating their labs.

Just don't create your starter on your work stir plate and you should be fine! My E.coli from my work that contains a plasmid with beta-amylase hasn't contaminated my brewing yet...but that might just increase my efficiency:p

Heh Heh, that was my major concern, using the lab shaking incubators. Talked to the bossman, incubators are not sterile environments and it's up to us as researchers to keep things out of their samples!

brewguyver 08-14-2012 11:49 AM

Quote:

Originally Posted by ScoRas

We even have a hard time convincing the flow cytometry core to run our live yeast surface-display samples.

A little off topic -

My brewing partner (product engineer) is in process of building a flow cytometer from high end microscopy parts that his company scrapped. I've been "assigned" the job of figuring out yeast assays. Right now I'm trying to figure out ways to differentiate (fingerprint) different yeast strains/identify presence of contaminants in a sample, but I'm also interested in any suggestions around vitality or viability studies.

It would be good to discuss ideas with biologists who also brew beer. Would one of you folks be up for a little correspondence?

broadbill 08-14-2012 12:14 PM

Quote:

Originally Posted by brewguyver (Post 4329205)
A little off topic -

My brewing partner (product engineer) is in process of building a flow cytometer from high end microscopy parts that his company scrapped. I've been "assigned" the job of figuring out yeast assays. Right now I'm trying to figure out ways to differentiate (fingerprint) different yeast strains/identify presence of contaminants in a sample, but I'm also interested in any suggestions around vitality or viability studies.

It would be good to discuss ideas with biologists who also brew beer. Would one of you folks be up for a little correspondence?

You guys are actually building a flow cytometer for analyzing brewing yeast strains? If so, you guys have definitely won the award for "going overboard" on this homebrewing things!!...and I mean that in the best possible way.:mug:

I'd be interested to hear what you are up to...you could say I'm a biologist who brews beer!:D

smokinghole 08-18-2012 02:11 PM

I was allowed to do yeast starters in the shaker incubator at school with no worry about contaminating the bacterial cells we kept on hand. It was up to the profs and students to keep contamination out of their samples and work. Unless you're working in a clean room of sorts with HEPA and air locks I don't see how you bringing it in would cause anyone problems. Specific pieces of equipment that are only really used for one type of cell could pose a problem I guess but no more than airborne contamination if you ask me.

Brewitt 08-28-2012 07:41 PM

There are low levels of bacteria, molds and fungi in the air, surfaces in the environment and certainly on your body. I presume, if you use proper lab technique you are not getting a lot of contamination of your cultures. Furthermore, most yeast molecular biologists and geneticists use E.coli extensively in their laboratories and, if not their own, certainly the labs around them do. Hence the need for sterile technique. If your stocks aren't constantly becoming contaminated with E coli, which are easy to see because they grow quickly and take over a culture, they are unlikely to be getting cross contaminated with other yeast. In my experience, it is relatively uncommon to get different laboratory strains cross contaminated except by making the mistake of mixing them. We work regularly with hundreds of strains and multiple species of yeast and don't have a problem. Just be careful.

The other issue brought up here is contamination of mammalian cells with yeast. Saccharomyces cerevisiae doesnt grow well under conditions for tissue culture. While I would not say they can't grow, I don't think they grow well. I believe that most fungal contamination of tissue culture that are observed are from other, non-laboratory, species.

Finally, although it is admirable to make a homemade flow cytometer (wow!), I don't think you will have much luck identifying different yeast strains using that instrument unless you have some way of staining cells for distinct features or have very good resolution to distinguish morphologies (even with that you will have to know what distiguishes the strains which is not well documented). Or, perhaps I misunderstood the goal of the project. That said, have fun with the project.

brewguyver 08-30-2012 03:34 AM

Yea - buddy is a product engineer for a med devices company. They scrapped a bunch of high-end-but-outdated equipment (bad quarter?) and he picked it up for a song. I think the end game is a little more ambitious than cell-counting overkill, but we have to start somewhere.

We do have the capability to do staining, have a full UV rig for the scopes, etc and a few microbiologist friends who will "work" for beer. However, I'd love to get some input from you guys. Shoot over suggestions and we'll try them out, report back, and even snap some photos.


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