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Old 03-07-2009, 03:11 AM   #51
Schlenkerla
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Yeah, this is really cool. What did you mix the agar with for the culture medium? Malt extract?
Yes - I used light dme. http://brewiki.org/Yeast/Culturing#head-062dddbe5c00d419ef9f0931a737f4bfeaca3394

My first open exposure was exactly 60 minutes. I have my closed sample incubating at 68F. I'll peek at in about 24hrs.


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Old 03-07-2009, 09:11 AM   #52
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Looks good! As a non-microbiologist, what's the advantage of using agar rather than normal wort?



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Old 03-07-2009, 03:42 PM   #53
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Looks good! As a non-microbiologist, what's the advantage of using agar rather than normal wort?
Let say I go out and collect a sample of yeast and its possible get some mold too along with the yeast.

If I have the agar sample I can watch surface growth right on the agar. The agar specimen is a hard DME jelly. DME boiled with agar.

Look at this picture. The white streaks are yeast and other gray/green stuff is mold.



I can use a sterile loop (SS wire heated via flame) to lift off some yeast to place into a 25 or 50ml starter. Then start stepping it up to a larger size.

If I used a jar of wort I could get both mold & yeast. The fermented sample might be infected or plain nasty. Its possible that I could be just fine as well.

The difference is that with AGAR I can select what I want to put in a starter. Mainly to avoid mold and have more control.


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Old 03-07-2009, 06:53 PM   #54
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This makes for a great/easy way to store yeast cultures too. Just take the yeast colonies you want (without any agar) and store them in deionized/distilled water in sterile tubes.

You know that you are getting yeast without any other 'bugs'

~M~

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Old 03-08-2009, 07:35 AM   #55
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Any growth yet, Schlenkerla?

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Old 03-08-2009, 03:13 PM   #56
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This makes for a great/easy way to store yeast cultures too. Just take the yeast colonies you want (without any agar) and store them in deionized/distilled water in sterile tubes.

You know that you are getting yeast without any other 'bugs'

~M~
That is what I do, but not distilled or DI. I use tap water that is sterilized. Stored in test tubes.

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Any growth yet, Schlenkerla?
No growth so far. I'm wondering if I got anything in the 60 minute open time. I will wait another 24 then take another go at collecting something. I'll increase the time to 4 hours.

Whatever I do it won't be over night. I don't want the condensation.
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Old 03-08-2009, 05:44 PM   #57
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That is what I do, but not distilled or DI. I use tap water that is sterilized. Stored in test tubes.



No growth so far. I'm wondering if I got anything in the 60 minute open time. I will wait another 24 then take another go at collecting something. I'll increase the time to 4 hours.

Whatever I do it won't be over night. I don't want the condensation.
I wonder if there is a way around the condensation? Take it in every night?
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Old 03-08-2009, 07:04 PM   #58
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The difference is that with AGAR I can select what I want to put in a starter. Mainly to avoid mold and have more control.
Cool! Thanks for the explanation.
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Old 03-08-2009, 08:37 PM   #59
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I wonder if there is a way around the condensation? Take it in every night?
Yes - Hopefully it take only an evening/afternoon.
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Old 03-08-2009, 08:57 PM   #60
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Schlenkerla, have you considered using a loop to innoculate a plate with wild samples. For instance, I was thinking about using a loop to collect some nectar from a bloom on a meyer lemon tree I have to see if there were any wild yeasts living in there.



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