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Old 10-01-2009, 02:42 PM   #281
Ryan_PA
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I just started my version of this experiment as well. My plan is to take the wild yeast, and streak some on a agar plate, trying to isolate the yeast colonies from the other microbs. Then, I want to do a test batch with split fermentation, one with the wild cocktail, and one with an isolated yeast colony build up from the plate. I hope to let both age fairly long, and sample down the road side by side.

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Old 10-02-2009, 01:13 AM   #282
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I think I will follow this agar plate method as well. At one point I had what seemed like a good layer of yeast sediment in my mason jars a day or so before the mold kicked in and ruined the batch. That appears to be the best time to take a sample.

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Old 10-02-2009, 12:11 PM   #283
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How many days in would you say that was?

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Old 10-02-2009, 07:02 PM   #284
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It was about 3-4 days. Within two more I started growing mold, so it was a pretty short window. It was my first effort though, so who knows... Gonna set up and try again tonight..

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Old 10-02-2009, 07:24 PM   #285
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Cool man, thanks. I will let you know how my attempt goes. I put out my wort on Wednesday so I will check on it tonight. If there is any sign of ferment, I am going to cover with aluminum foil and put in a draft free area.

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Old 10-08-2009, 12:41 AM   #286
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So I let the wort sit out for about 4 days, and plated a few streaks on agar. It grew for about 3 days, and there are defined colonies. I see some white (what I am believing is yeast) colonies, a definite mold growth, and some funky reddish/orange growths. Anyone have any idea what the red/orange circles could be?

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Old 10-08-2009, 02:55 PM   #287
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Awesome thread, and awesome results.

Ryan PA, can you take a picture of your agar plate? I could have a look if you want.

I did the experiment too - poured some plates, containing

-10g yeast extract
-20g peptone
-20g maltose
-20g agar
-1L water

About 100ml per plate (big ones)

Left outside overnight, closed plate in the morning, grew at room temperature. After 2 days colonies started to appear. Most of them were filamentous molds, but also some white, shiny colonies. Picked colonies, checked them under stereo microscope, looked promising. Grew them in the same media overnight, but without agar and shaking in little erlenmeyer flasks at 25C.

checked under light microscope, and they were all bacterial :/

Interesting to see what was there, all kinds of stuff, but no yeast

Next try will be more serious. I think what would be good is to use indeed a media which is not too rich as I did. Maybe something which is selective. I am not a yeast expert at all but I am quite familiar with doing microbiological work. Any tips on plates which are a little selective?

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Old 10-08-2009, 03:08 PM   #288
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Cool man, I will try to get a pic tomorrow when I am back home. I added hops to my wort in an attempt to promote yeast over bugs, but I figured that may be a non-point.

I left the plate out a little longer, to see if I get any more colonies.

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Old 10-09-2009, 03:15 AM   #289
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I definitely went over the top with those sugars. Next try will be less half-assed. I will also wait until it cools down more, bacteria will outgrow anything now here. Just a few more weeks, then more minimal media (no sugar, maybe just soluble starch or something), and maybe a trial with fruits thrown in.

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Old 10-14-2009, 11:39 PM   #290
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Quote:
Originally Posted by Ryan_PA View Post
Cool man, I will try to get a pic tomorrow when I am back home. I added hops to my wort in an attempt to promote yeast over bugs, but I figured that may be a non-point.

I left the plate out a little longer, to see if I get any more colonies.
any pics yet?
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