Just had a very nice email conversation with the guys from Wyeast and though it would be good to share it:
"Me: I just bought 2 packs of 3278 - Belgian Lambic BlendT propagators and don't know how to use them. Can I pitch them straight into a 5gal batch? Do I have to make a starter? If so, how and how long should it sit before pitching?
The 3278 Activator pack is designed to be directly pitched into 5 gallons of wort. I do not recommend making starts with this blend.
Please let me know if you have other questions.
Me:What about the Propagator (50ml) pack for this blend? Can I pitch it
straight to a 5 bal batch or should I use 2 packs?
Can I re-use it after transfering to a 2ary fermenter?
Wyeast:Sorry... misread the e-mail. Use two Propagators for 5 gallons.
You can reuse the yeast/bacteria, but the resulting beer will have a different profile due to the alerted ratios of Brett:Saccharomyces:bacteria.
Me:One last question. When reusing if I pitch a pure Saccharomides before pitch the old blend can I get similar results? Or the Bret/Pedio/Lacto proportions will also be different?
Wyeast:You will still get an altered flavor profile either way.
Me:OK. Thanks for your help.
I'll tell you in a year how it turns out.
Wyeast:Thanks. I look forward to hearing back from you!"