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Old 02-25-2010, 02:07 AM   #1
JacobS
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Default Seeking Advise on yeast culturing

I am about to attempt to culture yeast out of a couple of bottles of beer. I would like to streak plates and work from there. I have never done this before and would be interested in any thoughts and experience out there.

Also just to make it even harder on me I am going to be attempting to do this from spontaneously fermented beers. (Yes I am aware of the microbial soup that they contain) My thought/ hope is to pull one or two nice Sac. sp. yeast from them. So ...?

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Old 02-25-2010, 05:01 PM   #2
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Try posting on the BBB ( http://www.babblebelt.com/newboard/f...108752780&pg=1 ) you might get a better respose there for a complex microbe question like this. Personally I would just pitch the dregs along with a standard yeast culture, I doubt the Sacch from a lambic is that interesting/important.

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Old 02-25-2010, 05:24 PM   #3
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If you are after the yeast, I'd pitch them into a starter medium and let them grow some, then decant. You should be left with a higher percentage of yeast to work with.

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Old 03-01-2010, 04:19 AM   #4
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you would be better going with a slant to sub culture your yeast

any asian or phillipene store can provide malt agar (as they use this to cook with) just bring it to a boil then pour it into sterile tubes and lean them on a book about an inch thick

when time to sub culture have a flame sourse ready

sterile tech

flame the hoop

flame the tube

smear the sample

flame the tube

cap the tube

place in room temp incubation

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Old 03-01-2010, 06:26 AM   #5
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Thats about what I was thinking. Since I have an impure culture to begin with I was going to use petri dishes to find "Yeast' vs "beast' first Thank you

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