Originally Posted by ThaDutchMasta
wouldn't that result in an unsterilized medium?
do you inoculate your plates/slants in front of a flowhood or in open air?
I'm just talking about chilling the DME and water. I'd decant the wort off the break, bring to a boil and then add the agar and proceed with the pressure-cooker "auto-claving" and pouring.
Even without the auto-claving, there should only be a slight chance of contamination if my technique is good. But I'd rather play it safe.
I inoculate in open air, but close all windows and doors. I also turn off fans and make sure the dog is in another room.
My sterile technique also involves keeping the propane torch burner on at all times to flaming the mouths of test tubes, sterilize the loop, working in the flame area, etc.
It may be overkill, but I also wear a cheap painters mask... the kind you can get 5 for $5 at most hardware stores. This keeps my direct breath off the equipment and baffles my breath air currents.
SWMBO sure thinks the mask increases the mad scientist factor (MSF) even though all of the tubes and plates, etc generate a pretty high MSF already!
Is it possible that I am being way too careful? I'm sure of it!
Here's a quick rundown of sterile technique, if you're not familiar with it:http://structbio.vanderbilt.edu/chaz...o/sterile.html