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Old 06-30-2010, 03:56 AM   #1
beesy
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Default Newest addition - Autoclave!!

Yea me!!! I was lucky enough to venture out with our HR director to cut a lock off a storage unit we had apparently been paying for for around 5 years since our group parted ways with the university. Lucky for me in the back corner sat a lonely autoclave. Lonely that is, until I brought it home with me. Fired it up and it works like new. Exciting!! Now to find some autoclavable yeast containers at one of our offices!!

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Old 06-30-2010, 04:05 AM   #2
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Nice score!

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Old 06-30-2010, 12:32 PM   #3
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Just curious, what are you using for the pressurizing?

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Old 06-30-2010, 07:37 PM   #4
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Quote:
Originally Posted by Bubba_Mustafa View Post
Just curious, what are you using for the pressurizing?
an autoclave builds the pressure itself...and the OP needs to find autoclavable containers for storing yeast...

or did I misunderstand your question?

*edit* perhaps you wondered if he's doing a chemical additive or not? I know the autoclave pouches we use at the tattoo shop are both for steam/heat and chemical sterilization (two different color change indicators on the pouch).

should only need steam autoclave for yeast banking.
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Old 06-30-2010, 07:39 PM   #5
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any pitchers of the 'clave??

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Old 06-30-2010, 07:54 PM   #6
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Nice score!

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Old 07-01-2010, 02:50 AM   #7
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Quote:
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any pitchers of the 'clave??
yep - got one somewhere. let me get it uploaded. it's just a smaller lab top one with like a 9-10" mouth and approx 18" deep, but perfect for yeast banking materials. let me find a pic and get right back. it does have 4 modes - 2 with 121C and 128 kPa and 2 with 132C and 210kPa.

Yes you're correct - i just needs distilled H2O
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Old 07-01-2010, 02:56 AM   #8
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Quote:
Originally Posted by malkore View Post
an autoclave builds the pressure itself...and the OP needs to find autoclavable containers for storing yeast...

or did I misunderstand your question?

*edit* perhaps you wondered if he's doing a chemical additive or not? I know the autoclave pouches we use at the tattoo shop are both for steam/heat and chemical sterilization (two different color change indicators on the pouch).

should only need steam autoclave for yeast banking.
We have some smaller 100ml vessels for sending out tissue samples at our offices that are autoclavable (and they come sterilized). Just need to get out and get a few. at this time wasn't planning on doing glycol frozen banks. i need to get my condo done and get a clean room built in the basement first - then we'll talk glycol based banks
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Old 07-01-2010, 03:04 AM   #9
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any pitchers of the 'clave??
here ya' go

clave 1

clave 2
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Old 07-01-2010, 03:13 AM   #10
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Wow that is totally sweet.
I'm jealous.

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